WB (Western Blot)
(Western blot analysis Separase using 293 whole cell lysates)
Rabbit anti-Human Separase Polyclonal Antibody | anti-SSE antibody
Separase Antibody
Gene Names
ESPL1; ESP1; SEPA
Reactivity
Human
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
Separase, Antibody; Separase Antibody; Caspase like protein ESPL1; Caspase-like protein ESPL1; ESP 1; ESP1; ESPL 1; Espl1; ESPL1_HUMAN; Extra spindle poles like 1; Extra spindle poles like 1 protein; Extra spindle poles-like 1 protein; FLJ46492; KIAA0165; Separase; Separin; Similar to fission yeast cut1and gene; anti-SSE antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Separase antibody detects endogenous levels of total Separase
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
2120
Applicable Applications for anti-SSE antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide derived from human Separase
Subcellular Location
Cytoplasm. Nucleus.
Predicted Cross Reactivity
Sheep, Rabbit, Dog
Similarity
Sheep (80%), Rabbit (90%), Dog (90%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis Separase using 293 whole cell lysates)
WB (Western Blot)
(Western blot analysis Separase using 293 whole cell lysates)
IF (Immunofluorescence)
(AAA322622 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IF (Immunofluorescence)
(AAA322622 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-SSE antibody
Function: Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.
Subunit Structure: Interacts with PTTG1. Interacts with RAD21.
Post-translational Modifications: Autocleaves. This function, which is not essential for its protease activity, is unknown. Phosphorylated by CDK1. There are 8 Ser/Thr phosphorylation sites. Among them, Ser-1126 phosphorylation is the major site, which conducts to the enzyme inactivation.
Subunit Structure: Interacts with PTTG1. Interacts with RAD21.
Post-translational Modifications: Autocleaves. This function, which is not essential for its protease activity, is unknown. Phosphorylated by CDK1. There are 8 Ser/Thr phosphorylation sites. Among them, Ser-1126 phosphorylation is the major site, which conducts to the enzyme inactivation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 230 kDa
Predicted: 234 kDa
Predicted: 234 kDa
NCBI Official Full Name
separin
NCBI Official Synonym Full Names
extra spindle pole bodies like 1, separase
NCBI Official Symbol
ESPL1
NCBI Official Synonym Symbols
ESP1; SEPA
NCBI Protein Information
separin
UniProt Protein Name
Separin
UniProt Gene Name
ESPL1
UniProt Synonym Gene Names
ESP1; KIAA0165
Similar Products
Product Notes
The SSE espl1 (Catalog #AAA322622) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Separase Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Separase can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the SSE espl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Separase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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