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product-image-AAA211819_WB8.jpg WB (Western Blot) (Detection of human SET by western blot. Samples: Whole cell lysate (10 ug) from Hep-G2, HeLa, Jurkat, MCF-7, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SET antibody AAA211819 (lot AAA211819-3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

Rabbit anti-Human, Mouse SET Polyclonal Antibody | anti-SET antibody

Rabbit anti-SET Antibody, Affinity Purified

Gene Names
SET; 2PP2A; IGAAD; TAF-I; I2PP2A; IPP2A2; PHAPII; TAF-IBETA
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
SET, Antibody; Rabbit anti-SET Antibody, Affinity Purified; I2PP2A; protein phosphatase type 2A inhibitor; protein SET; SET nuclear oncogene; SET translocation (myeloid leukemia-associated); TAF-I; TAF-IBETA; Template-activating factor I; phosphatase 2A inhibitor I2PP2A; Template-Activating Factor-I, chromatin remodelling factor; MRD58; IPP2A2; inhibitor-2 of protein phosphatase-2A; inhibitor of granzyme A-activated DNase; I-2PP2A; IGAAD; HLA-DR-associated protein II; chromatin remodelling factor; 2PP2A; PHAPII; Protein SET; SET nuclear proto-oncogene; anti-SET antibody
Ordering
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-SET antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat
Immunogen
Between 150 and 200
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human SET by western blot. Samples: Whole cell lysate (10 ug) from Hep-G2, HeLa, Jurkat, MCF-7, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SET antibody AAA211819 (lot AAA211819-3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

product-image-AAA211819_WB8.jpg WB (Western Blot) (Detection of human SET by western blot. Samples: Whole cell lysate (10 ug) from Hep-G2, HeLa, Jurkat, MCF-7, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SET antibody AAA211819 (lot AAA211819-3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

WB (Western Blot)

(Detection of mouse SET by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SET antibody AAA211819 (lot AAA211819-3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)

product-image-AAA211819_WB10.jpg WB (Western Blot) (Detection of mouse SET by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SET antibody AAA211819 (lot AAA211819-3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)

IP (Immunoprecipitation)

(Detection of human SET by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SET antibody AAA211819 (lot AAA211819-3) used for IP at 6 ug per reaction. SET was also immunoprecipitated by a previous lot of this antibody (lot AAA211819-2) and rabbit anti-SET antibody For blotting immunoprecipitated SET, AAA211819 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)

product-image-AAA211819_IP11.jpg IP (Immunoprecipitation) (Detection of human SET by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SET antibody AAA211819 (lot AAA211819-3) used for IP at 6 ug per reaction. SET was also immunoprecipitated by a previous lot of this antibody (lot AAA211819-2) and rabbit anti-SET antibody For blotting immunoprecipitated SET, AAA211819 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)

IHC (Immunohiostchemistry)

(Detection of mouse SET by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-SET (Cat. No. AAA211819 Lot3) used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB)

product-image-AAA211819_IHC13.jpg IHC (Immunohiostchemistry) (Detection of mouse SET by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-SET (Cat. No. AAA211819 Lot3) used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB)

IHC (Immunohistochemistry)

(Detection of human SET by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-SET (Cat. No. AAA211819 Lot3) used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB)

product-image-AAA211819_IHC15.jpg IHC (Immunohistochemistry) (Detection of human SET by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-SET (Cat. No. AAA211819 Lot3) used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-SET antibody
SET is a multi-functional protein involved in apoptosis, transcription, nucleosome assembly, and histone binding. SET was identified as part of a chromosomal rearrangement with NUP214/CAN in acute undifferentiated leukemia.
Product Categories/Family for anti-SET antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
31,272 Da
NCBI Official Full Name
protein SET isoform 1
NCBI Official Synonym Full Names
SET nuclear proto-oncogene
NCBI Official Symbol
SET
NCBI Official Synonym Symbols
2PP2A; IGAAD; TAF-I; I2PP2A; IPP2A2; PHAPII; TAF-IBETA
NCBI Protein Information
protein SET; HLA-DR-associated protein II; SET nuclear oncogene; SET translocation (myeloid leukemia-associated); Template-Activating Factor-I, chromatin remodelling factor; inhibitor of granzyme A-activated DNase; inhibitor-2 of protein phosphatase-2A; p
UniProt Protein Name
Protein SET
UniProt Gene Name
SET
UniProt Synonym Gene Names
IGAAD; I-2PP2A; TAF-I
UniProt Entry Name
SET_HUMAN

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Product Notes

The SET set (Catalog #AAA211819) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-SET Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SET can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SET set for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SET, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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