WB (Western Blot)
(Western blot analysis of SETDB1(arrow) using rabbit polyclonal SETDB1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SETDB1 gene (Lane 2) (Origene Technologies).)
Rabbit SETDB1 Polyclonal Antibody | anti-SETDB1 antibody
SETDB1 Antibody (C-term)
Gene Names
SETDB1; ESET; KG1T; KMT1E; TDRD21; H3-K9-HMTase4
Reactivity
Human (Predicted Reactivity: Mouse, Xenopus, Zebrafish)
Applications
ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SETDB1, Antibody; SETDB1 Antibody (C-term); Histone-lysine N-methyltransferase SETDB1; ERG-associated protein with SET domain; ESET; Histone H3-K9 methyltransferase 4; H3-K9-HMTase 4; Lysine N-methyltransferase 1E; SET domain bifurcated 1; SETDB1; KIAA0067; KMT1E; anti-SETDB1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Xenopus, Zebrafish)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This SETDB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1193-1225 amino acids from the C-terminal region of human SETDB1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
1193-1225
Sequence Length
1291
Applicable Applications for anti-SETDB1 antibody
ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of SETDB1(arrow) using rabbit polyclonal SETDB1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SETDB1 gene (Lane 2) (Origene Technologies).)
WB (Western Blot)
(Western blot analysis of SETDB1(arrow) using rabbit polyclonal SETDB1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SETDB1 gene (Lane 2) (Origene Technologies).)
WB (Western Blot)
(Western blot analysis of anti-SETDB1 Pab in Ramos cell line lysate (35ug/lane). SETDB1(arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of anti-SETDB1 Pab in Ramos cell line lysate (35ug/lane). SETDB1(arrow) was detected using the purified Pab.)
Related Product Information for anti-SETDB1 antibody
The SET domain is a highly conserved, approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger conserved region present in the Drosophila Trithorax protein and was subsequently identified in the Drosophila Su(var)3-9 and 'Enhancer of zeste' proteins, from which the acronym SET is derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities.
References
Ichimura, T., et al., J. Biol. Chem. 280(14):13928-13935 (2005).
Sarraf, S.A., et al., Mol. Cell 15(4):595-605 (2004).
Wang, H., et al., Mol. Cell 12(2):475-487 (2003).
Schultz, D.C., et al., Genes Dev. 16(8):919-932 (2002).
Yang, L., et al., Biochem. J. 369 (PT 3), 651-657 (2003) (): ().
Sarraf, S.A., et al., Mol. Cell 15(4):595-605 (2004).
Wang, H., et al., Mol. Cell 12(2):475-487 (2003).
Schultz, D.C., et al., Genes Dev. 16(8):919-932 (2002).
Yang, L., et al., Biochem. J. 369 (PT 3), 651-657 (2003) (): ().
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
143157
NCBI Official Full Name
histone-lysine N-methyltransferase SETDB1 isoform 1
NCBI Official Synonym Full Names
SET domain, bifurcated 1
NCBI Official Symbol
SETDB1
NCBI Official Synonym Symbols
ESET; KG1T; KMT1E; TDRD21; H3-K9-HMTase4
NCBI Protein Information
histone-lysine N-methyltransferase SETDB1
UniProt Protein Name
Histone-lysine N-methyltransferase SETDB1
UniProt Gene Name
SETDB1
UniProt Synonym Gene Names
KIAA0067; KMT1E; ESET; H3-K9-HMTase 4
UniProt Entry Name
SETB1_HUMAN
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Product Notes
The SETDB1 setdb1 (Catalog #AAA288277) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1193-1225. The SETDB1 Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse, Xenopus, Zebrafish) and may cross-react with other species as described in the data sheet. AAA Biotech's SETDB1 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the SETDB1 setdb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SETDB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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