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IHC (Immunohistochemistry)
(Anti- SF2 Picoband antibody, AAA46370, IHC(P)IHC(P): Human Mammary Cancer Tissue)
SF2 Polyclonal Antibody | anti-SF2 antibody
Anti-SF2 Antibody
Gene Names
SRSF1; ASF; SF2; SFRS1; SF2p33; SRp30a
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Synonyms
SF2, Antibody; Anti-SF2 Antibody; Serine/arginine-rich splicing factor 1; Alternative splicing factor 1; Alternative-splicing factor 1; arginine/serine-rich 1; ASF 1; ASF; ASF-1; ASF1; FLJ53078; MGC5228; P33 subunit; Pre mRNA splicing factor SF2 P33 subunit; pre-mRNA-splicing factor SF2; SF2; SF2P33; SFRS1; Splicing factor 2 alternate splicing factor; Splicing factor 2; Splicing factor; Splicing factor arginine/serine rich 1; SR Splicing factor 1; SRp30a; srsf1; SRSF1_HUMAN; serine/arginine-rich splicing factor 1; anti-SF2 antibody
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
201
Applicable Applications for anti-SF2 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of Human SF2(6-33aa VIRGPAGNNDCRIYVGNLPPDIRTKDIE), identical to the related mouse sequence.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohistochemistry)
(Anti- SF2 Picoband antibody, AAA46370, IHC(P)IHC(P): Human Mammary Cancer Tissue)
IHC (Immunohistochemistry)
(Anti- SF2 Picoband antibody, AAA46370, IHC(P)IHC(P): Human Mammary Cancer Tissue)
IHC (Immunohistochemisry)
(Anti- SF2 Picoband antibody, AAA46370, IHC(P)IHC(P): Rat Brain Tissue)
IHC (Immunohistochemisry)
(Anti- SF2 Picoband antibody, AAA46370, IHC(P)IHC(P): Rat Brain Tissue)
IHC (Immunohiostchemistry)
(Anti- SF2 Picoband antibody, AAA46370, IHC(P)IHC(P): Mouse Brain Tissue)
IHC (Immunohiostchemistry)
(Anti- SF2 Picoband antibody, AAA46370, IHC(P)IHC(P): Mouse Brain Tissue)
WB (Western Blot)
(Anti- SF2 Picoband antibody, AAA46370, Western blottingAll lanes: Anti SF2 (AAA46370) at 0.5ug/mlLane 1: Rat Testis Tissue Lysate at 50ugLane 2: Rat Brain Tissue Lysate at 50ugLane 3: NEURO Whole Cell Lysate at 40ugLane 4: 293T Whole Cell Lysate at 40ugLane 5: SW620 Whole Cell Lysate at 40ugPredicted bind size: 28KDObserved bind size: 28KD)
WB (Western Blot)
(Anti- SF2 Picoband antibody, AAA46370, Western blottingAll lanes: Anti SF2 (AAA46370) at 0.5ug/mlLane 1: Rat Testis Tissue Lysate at 50ugLane 2: Rat Brain Tissue Lysate at 50ugLane 3: NEURO Whole Cell Lysate at 40ugLane 4: 293T Whole Cell Lysate at 40ugLane 5: SW620 Whole Cell Lysate at 40ugPredicted bind size: 28KDObserved bind size: 28KD)
Related Product Information for anti-SF2 antibody
Description: Rabbit IgG polyclonal antibody for Serine/arginine-rich splicing factor 1(SRSF1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Background: SF2, also known as Serine/arginine-rich splicing factor 1 (SRSF1), is a protein that in humans is encoded by the SFRS1 gene. This gene encodes a member of the arginine/serine-rich splicing factor protein family. There is a pseudogene of this gene on chromosome 13. The encoded protein can either activate or repress splicing, depending on its phosphorylation state and its interaction partners. Multiple transcript variants have been found for this gene. ASF/SF2 is necessary for all splicing reactions to occur, and influences splice site selection in a concentration-dependent manner, resulting in alternative splicing.
Background: SF2, also known as Serine/arginine-rich splicing factor 1 (SRSF1), is a protein that in humans is encoded by the SFRS1 gene. This gene encodes a member of the arginine/serine-rich splicing factor protein family. There is a pseudogene of this gene on chromosome 13. The encoded protein can either activate or repress splicing, depending on its phosphorylation state and its interaction partners. Multiple transcript variants have been found for this gene. ASF/SF2 is necessary for all splicing reactions to occur, and influences splice site selection in a concentration-dependent manner, resulting in alternative splicing.
References
1. "Entrez Gene: SFRS1 splicing factor, arginine/serine-rich 1 (splicing factor 2, alternate splicing factor)". 2. Michlewski G, Sanford JR, Cáceres JF (April 2008). "The splicing factor SF2/ASF regulates translation initiation by enhancing phosphorylation of 4E-BP1". Mol. Cell 30 (2): 179-89. 3. Zuo P, Manley JL (December 1993)."Functional domains of the human splicing factor ASF/SF2". EMBO J. 12 (12): 4727-37.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,460 Da
NCBI Official Full Name
serine/arginine-rich splicing factor 1 isoform 2
NCBI Official Synonym Full Names
serine/arginine-rich splicing factor 1
NCBI Official Symbol
SRSF1
NCBI Official Synonym Symbols
ASF; SF2; SFRS1; SF2p33; SRp30a
NCBI Protein Information
serine/arginine-rich splicing factor 1
UniProt Protein Name
Serine/arginine-rich splicing factor 1
UniProt Gene Name
SRSF1
UniProt Synonym Gene Names
ASF; SF2; SF2P33; SFRS1; ASF-1
UniProt Entry Name
SRSF1_HUMAN
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Product Notes
The SF2 srsf1 (Catalog #AAA46370) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-SF2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SF2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the SF2 srsf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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