WB (Western Blot)
(Detection of human and mouse SF3A3 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SF3A3 antibody AAA211903 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. SF3A3 was also immunoprecipitated by rabbit anti-SF3A3 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).)
Rabbit anti-Human, Mouse SF3A3 Polyclonal Antibody | anti-SF3A3 antibody
Rabbit anti-SF3A3 Antibody, Affinity Purified
Gene Names
SF3A3; PRP9; PRPF9; SAP61; SF3a60
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
SF3A3, Antibody; Rabbit anti-SF3A3 Antibody, Affinity Purified; spliceosome associated protein 61; splicing factor 3a, subunit 3, 60kDa; splicing factor 3a, subunit 3, 60kD; pre-mRNA splicing factor SF3a (60kD); Spliceosome-associated protein 61; SF3a60; SAP61; SAP 61; PRPF9; PRP9; splicing factor 3A subunit 3; Splicing factor 3A subunit 3; splicing factor 3a subunit 3; anti-SF3A3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-SF3A3 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 451 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse SF3A3 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SF3A3 antibody AAA211903 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. SF3A3 was also immunoprecipitated by rabbit anti-SF3A3 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).)
WB (Western Blot)
(Detection of human and mouse SF3A3 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SF3A3 antibody AAA211903 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. SF3A3 was also immunoprecipitated by rabbit anti-SF3A3 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human SF3A3 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-SF3A3 (Cat. No. AAA211903 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human SF3A3 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-SF3A3 (Cat. No. AAA211903 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-SF3A3 antibody
SF3A3 is one of 3 subunits of the SF3a splicing factor. SF3a is composed of a 60 kDa subunit (SF3A3/SF3a60), 66 kDa subunit (SF3A2/SF3a66), and a120 kDa subunit (SF3A1/SF3a120). SF3a is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing [taken from NCBI Entrez Gene (GeneID: 10946)].
Product Categories/Family for anti-SF3A3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
58,849 Da
NCBI Official Full Name
splicing factor 3A subunit 3
NCBI Official Synonym Full Names
splicing factor 3a, subunit 3, 60kDa
NCBI Official Symbol
SF3A3
NCBI Official Synonym Symbols
PRP9; PRPF9; SAP61; SF3a60
NCBI Protein Information
splicing factor 3A subunit 3; SAP 61; spliceosome associated protein 61; spliceosome-associated protein 61; splicing factor 3a, subunit 3, 60kD; pre-mRNA splicing factor SF3a (60kD)
UniProt Protein Name
Splicing factor 3A subunit 3
UniProt Gene Name
SF3A3
UniProt Synonym Gene Names
SAP61; SAP 61
UniProt Entry Name
SF3A3_HUMAN
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Product Notes
The SF3A3 sf3a3 (Catalog #AAA211903) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-SF3A3 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SF3A3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SF3A3 sf3a3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SF3A3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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