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product-image-AAA282215_IF10.jpg IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using [KD Validated] SFRP2 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

Rabbit anti-Human SFRP2 Polyclonal Antibody | anti-SFRP2 antibody

[KD Validated] SFRP2 Rabbit pAb

Gene Names
LOC4332059; Lhs1; MADS1; OsMADS1
Reactivity
Human
Applications
Western Blot
Purity
Affinity purification
Synonyms
SFRP2, Antibody; [KD Validated] SFRP2 Rabbit pAb; SFRP2; FRP-2; SARP1; SDF-5; anti-SFRP2 antibody
Ordering
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Applicable Applications for anti-SFRP2 antibody
WB (Western Blot)
Positive Samples
HeLa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SFRP2 (NP_003004.1).
Cellular Location
extracellular space
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.

IF (Immunofluorescence)

(Immunofluorescence analysis of PC-12 cells using [KD Validated] SFRP2 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

product-image-AAA282215_IF10.jpg IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using [KD Validated] SFRP2 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence)

(Immunofluorescence analysis of NIH/3T3 cells using [KD Validated] SFRP2 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

product-image-AAA282215_IF11.jpg IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using [KD Validated] SFRP2 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence)

(Immunofluorescence analysis of A431 cells using [KD Validated] SFRP2 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

product-image-AAA282215_IF13.jpg IF (Immunofluorescence) (Immunofluorescence analysis of A431 cells using [KD Validated] SFRP2 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

WB (Western Blot)

(Western blot analysis of extracts from wild type(WT) and SFRP2 knockdown (KD) HeLa cells, using SFRP2 antibody at 1:1616 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

product-image-AAA282215_WB15.jpg WB (Western Blot) (Western blot analysis of extracts from wild type(WT) and SFRP2 knockdown (KD) HeLa cells, using SFRP2 antibody at 1:1616 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Related Product Information for anti-SFRP2 antibody
This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. Methylation of this gene is a potential marker for the presence of colorectal cancer.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29,528 Da
NCBI Official Full Name
MADS-box transcription factor 1
NCBI Official Symbol
LOC4332059
NCBI Official Synonym Symbols
Lhs1; MADS1; OsMADS1
NCBI Protein Information
MADS-box transcription factor 1
UniProt Protein Name
MADS-box transcription factor 1
UniProt Gene Name
MADS1
UniProt Synonym Gene Names
LHS1

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Product Notes

The SFRP2 mads1 (Catalog #AAA282215) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The [KD Validated] SFRP2 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SFRP2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the SFRP2 mads1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SFRP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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