IF (Immunofluorescence)
(AAA328115 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit anti-Human SFRS14 Polyclonal Antibody | anti-SFRS14 antibody
SFRS14 Antibody
Gene Names
SUGP2; SFRS14; SRFS14
Reactivity
Human
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
SFRS14, Antibody; SFRS14 Antibody; Arginine/serine rich splicing factor 14; arginine/serine-rich 14; Arginine/serine-rich-splicing factor 14; DKFZp779L2418; KIAA0365; Splicing factor; Splicing factor, arginine/serine-rich 14; Sugp2; SUGP2_HUMAN; SURP and G-patch domain-containing protein 2; anti-SFRS14 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
SFRS14 antibody detects endogenous levels of total SFRS14
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1082
Applicable Applications for anti-SFRS14 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide derived from human SFRS14
Subcellular Location
Nucleus.
Tissue Specificity
Detected in adult testis, and in fetal brain and kidney.
Predicted Cross Reactivity
Bovine, Sheep
Similarity
Bovine (83%), Sheep (83%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA328115 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IF (Immunofluorescence)
(AAA328115 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis SFRS14 using HuvEc whole cell lysates)
WB (Western Blot)
(Western blot analysis SFRS14 using HuvEc whole cell lysates)
Related Product Information for anti-SFRS14 antibody
Function: May play a role in mRNA splicing.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 116 kDa
Predicted: 121 kDa
Predicted: 121 kDa
NCBI Official Full Name
SURP and G-patch domain-containing protein 2 isoform 1
NCBI Official Synonym Full Names
SURP and G-patch domain containing 2
NCBI Official Symbol
SUGP2
NCBI Official Synonym Symbols
SFRS14; SRFS14
NCBI Protein Information
SURP and G-patch domain-containing protein 2
UniProt Protein Name
SURP and G-patch domain-containing protein 2
UniProt Gene Name
SUGP2
UniProt Synonym Gene Names
KIAA0365; SFRS14
Similar Products
Product Notes
The SFRS14 sugp2 (Catalog #AAA328115) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SFRS14 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SFRS14 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the SFRS14 sugp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SFRS14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.