Rabbit SFRS2 Polyclonal Antibody | anti-SRSF2 antibody

SFRS2 Antibody (N-term)

Cat. Num. AAA290045

• Gene Names: SRSF2; SC35; PR264; SC-35; SFRS2; SFRS2A; SRp30b
• Reactivity: Human (Predicted Reactivity: Bovine, Chicken, Mouse, Pig, Rat)
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: SFRS2, Antibody; SFRS2 Antibody (N-term); Serine/arginine-rich splicing factor 2; Protein PR264; Splicing component; 35 kDa; Splicing factor SC35; SC-35; Splicing factor; arginine/serine-rich 2; SRSF2; SFRS2; anti-SRSF2 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Bovine, Chicken, Mouse, Pig, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This SFRS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-39 amino acids from the N-terminal region of human SFRS2.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

• Applicable Applications for anti-SRSF2 antibody: IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Function: Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway. The phosphorylated form (by SRPK2) is required for cellular apoptosis in response to cisplatin treatment.
• Cellular Location: Nucleus. Nucleus, nucleoplasm. Nucleus speckle. Note=Phosphorylation by SRPK2 provokes its redistribution from the nuclear specke to nucleoplasm.
• Antigen Region: 9-39
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistochemisry)

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SFRS2 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of anti-SFRS2 Antibody (N-term) (AAA290045) in K562 cell line lysates (35ug/lane).SFRS2(arrow) was detected using the purified Pab.)

WB (Western Blot)

(All lanes : Anti-SFRS2 Antibody (N-term) at 1:4000 dilution Lane 1: HepG2 whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-SRSF2 antibody

SFRS2 is necessary for the splicing of pre-mRNA. The protein is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. And it interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. It binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3' and can bind to beta-globin mRNA and commit it to the splicing pathway.

References

Merdzhanova,G., Cell Death Differ. 15 (12), 1815-1823 (2008) Solis,A.S., J. Biol. Chem. 283 (35), 23619-23626 (2008) Donev,R., Mol. Psychiatry 12 (7), 681-690 (2007) Sureau,A., Proc. Natl. Acad. Sci. U.S.A. 89 (24), 11683-11687 (1992)

NCBI and Uniprot Product Information

• NCBI GI #: 306482646
• NCBI GeneID: 6427
• NCBI Accession #: NP_001182356.1; NP_003007.2
• NCBI GenBank Nucleotide #: NM_001195427.1
• UniProt Accession #: Q01130; B3KWD5; B4DN89; H0YG49
• Molecular Weight: 25476
• NCBI Official Full Name: serine/arginine-rich splicing factor 2
• NCBI Official Synonym Full Names: serine/arginine-rich splicing factor 2
• NCBI Official Symbol: SRSF2
• NCBI Official Synonym Symbols: SC35; PR264; SC-35; SFRS2; SFRS2A; SRp30b
• NCBI Protein Information: serine/arginine-rich splicing factor 2
• UniProt Protein Name: Serine/arginine-rich splicing factor 2
• UniProt Gene Name: SRSF2
• UniProt Synonym Gene Names: SFRS2; SC-35
• UniProt Entry Name: SRSF2_HUMAN