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WB (Western Blot)
(WB Suggested Anti-SGK1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: HepG2 cell lysateSGK1 is supported by BioGPS gene expression data to be expressed in HepG2)
Rabbit SGK1 Polyclonal Antibody | anti-SGK1 antibody
SGK1 antibody - N-terminal region
Gene Names
SGK1; SGK
Reactivity
Tested Species Reactivity:Human, Mouse
Predicted Species Reactivity:
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Purified
Synonyms
SGK1, Antibody; SGK1 antibody - N-terminal region; anti-SGK1 antibody
Host
Rabbit
Reactivity
Tested Species Reactivity:
Human, Mouse
Predicted Species Reactivity:
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
Human, Mouse
Predicted Species Reactivity:
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
Clonality
Polyclonal
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration
0.5 mg/mL (varies by lot)
Sequence
Synthetic peptide located within the following region: ANPSPPPSPSQQINLGPSSNPHAKPSDFHFLKVIGKGSFGKVLLARHKAE
Sequence Length
431
Applicable Applications for anti-SGK1 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Homology
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 92%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human SGK1
Protein Size (# AA)
431 amino acids
Protein Interactions
AKT1S1; YWHAQ; YWHAZ; MAPT; MTOR; CDKN1B; RPTOR; NEDD4L; Tmem109; Rabac1; GADD45G; UBC; MAGEA1; HSP90AA1; TMEM184C; WNK1; WNK2; WNK4; RAF1; PDPK1; NEDD4; TSC22D3; STUB1; HSPA4; ELAVL1; COMMD1; SYVN1; SCNN1B; RICTOR; PDK1; YPK1; IKBKG; CAMKK1; KCNJ1; GSK3A
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(WB Suggested Anti-SGK1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: HepG2 cell lysateSGK1 is supported by BioGPS gene expression data to be expressed in HepG2)
WB (Western Blot)
(WB Suggested Anti-SGK1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: HepG2 cell lysateSGK1 is supported by BioGPS gene expression data to be expressed in HepG2)
WB (Western Blot)
(Lanes:Lane 1: 30ug mouse renal epithelial lysateLane 2: 30ug mouse renal epithelial lysateLane 3: 30ug mouse renal epithelial lysatePrimary Antibody Dilution:1:500Secondary Antibody:Anti-rabbit-HRPSecondary Antibody Dilution:1:2500Gene Name:SGK1Submitted by:Dr. JUNMING FAN,ECU)
WB (Western Blot)
(Lanes:Lane 1: 30ug mouse renal epithelial lysateLane 2: 30ug mouse renal epithelial lysateLane 3: 30ug mouse renal epithelial lysatePrimary Antibody Dilution:1:500Secondary Antibody:Anti-rabbit-HRPSecondary Antibody Dilution:1:2500Gene Name:SGK1Submitted by:Dr. JUNMING FAN,ECU)
IHC (Immunohiostchemistry)
(Rabbit Anti-SGK1 AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Liver TissueObserved Staining: Cytoplasm and plasma membrane in sinusoidsPrimary Antibody Concentration: 1:100Other Working Concentrations: N/ASecondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)
IHC (Immunohiostchemistry)
(Rabbit Anti-SGK1 AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Liver TissueObserved Staining: Cytoplasm and plasma membrane in sinusoidsPrimary Antibody Concentration: 1:100Other Working Concentrations: N/ASecondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)
IHC (Immunohistochemistry)
(Rabbit Anti-SGK1 AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Pineal TissueObserved Staining: Cytoplasmic in cell bodies and processes of pinealocytesPrimary Antibody Concentration: 1:100Other Working Concentrations: 1/600Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)
IHC (Immunohistochemistry)
(Rabbit Anti-SGK1 AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Pineal TissueObserved Staining: Cytoplasmic in cell bodies and processes of pinealocytesPrimary Antibody Concentration: 1:100Other Working Concentrations: 1/600Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)
Related Product Information for anti-SGK1 antibody
This is a rabbit polyclonal antibody against SGK1. It was validated on Western Blot using a cell lysate as a positive control.
Target Description: This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell sur
Target Description: This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell sur
Product Categories/Family for anti-SGK1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
49kDa
NCBI Official Full Name
serine/threonine-protein kinase Sgk1 isoform 1
NCBI Official Synonym Full Names
serum/glucocorticoid regulated kinase 1
NCBI Official Symbol
SGK1
NCBI Official Synonym Symbols
SGK
NCBI Protein Information
serine/threonine-protein kinase Sgk1
UniProt Protein Name
Serine/threonine-protein kinase Sgk1
UniProt Gene Name
SGK1
UniProt Synonym Gene Names
SGK
UniProt Entry Name
SGK1_HUMAN
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Product Notes
The SGK1 sgk1 (Catalog #AAA200638) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SGK1 antibody - N-terminal region reacts with Tested Species Reactivity: Human, Mouse Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's SGK1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the SGK1 sgk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: ANPSPPPSPS QQINLGPSSN PHAKPSDFHF LKVIGKGSFG KVLLARHKAE. It is sometimes possible for the material contained within the vial of "SGK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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