IP (Immunoprecipitation)
(SGK1 was immunoprecipitated using:Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-SGK1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-SGK1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 49 kDaObserved band size :49 kDa)
Rabbit anti-Human SGK1/SGK Polyclonal Antibody | anti-SGK1 antibody
Anti-SGK1/SGK Antibody, Rabbit Polyclonal
Gene Names
SGK1; SGK
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
SGK1/SGK, Antibody; Anti-SGK1/SGK Antibody, Rabbit Polyclonal; SGK1/SGK Antibody, Rabbit PAb, Antigen Affinity Purified; serum/glucocorticoid regulated kinase 1; Anti-SGK Antibody; anti-SGK1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human SGK1/SGK
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-SGK1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human SGK1/SGK
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human SGK1/SGK, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(SGK1 was immunoprecipitated using:Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-SGK1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-SGK1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 49 kDaObserved band size :49 kDa)
IP (Immunoprecipitation)
(SGK1 was immunoprecipitated using:Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-SGK1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-SGK1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 49 kDaObserved band size :49 kDa)
WB (Western Blot)
(Anti-SGK1 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:49 kDaObserved band size:49 kDa)
WB (Western Blot)
(Anti-SGK1 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:49 kDaObserved band size:49 kDa)
Related Product Information for anti-SGK1 antibody
Serum-and glucocorticoid-inducible kinase 1 (SGK1), a protein kinase, shares significant structural similarity with other members of the AGC protein kinase family. The inactive SGK1 structure lacks the alphaC helix and this unique feature makes it distinct from other protein kinases. SGK1 is ubiquitously expressed, which plays a pivotal role in the hormonal regulation of several ion channels, carriers, pumps, and transcription factors or regulators. SGK1 plays an important role in the absorption of Na+ and water through the Na+ channel in the apical membrane of tubular epithelial cells. SGK1 expression is modulated by cell stress and hormones, including gluco-and mineralocorticoids. Compelling evidence suggests that increased SGK1 expression or function is related to the pathogenic stress hypothesis of major depression.
References
Tang Z, et al. (2018) Serum and glucocorticoid-regulated kinase 1 (sgk1) is a predictor of poor prognosis in non-small cell lung cancer, and its dynamic pattern following treatment with sgk1 inhibitor and gamma-ray irradiation was elucidated. Oncol Rep 39 (3): 1505-1515.Martin-Fernandez B, et al. (2014) Relevance of sgk1 in structural, functional and molecular alterations produced by aldosterone in heart. Horm Mol Biol Clin Investig 18 (2): 53-61.Dattilo V, et al. (2020) The emerging role of sgk1 (serum-and glucocorticoid-regulated kinase 1) in major depressive disorder: Hypothesis and mechanisms. Front Genet 11 826.Akhoon BA, et al. (2019) Computational insights into the active structure of sgk1 and its implication for ligand design. Biochimie 165 57-66.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
48,942 Da
NCBI Official Full Name
serine/threonine-protein kinase Sgk1 isoform 2
NCBI Official Synonym Full Names
serum/glucocorticoid regulated kinase 1
NCBI Official Symbol
SGK1
NCBI Official Synonym Symbols
SGK
NCBI Protein Information
serine/threonine-protein kinase Sgk1; Sgk1 variant i3; serine/threonine protein kinase SGK; serum/glucocorticoid-regulated kinase 1
UniProt Protein Name
Serine/threonine-protein kinase Sgk1
UniProt Gene Name
SGK1
UniProt Synonym Gene Names
SGK
UniProt Entry Name
SGK1_HUMAN
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Product Notes
The SGK1 sgk1 (Catalog #AAA254375) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SGK1/SGK Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SGK1/SGK can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SGK1 sgk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SGK1/SGK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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