WB (Western Blot)
(Western blot analysis of SGMS2 (arrow) using rabbit polyclonal SGMS2 Antibody (C-term)(AAA283932). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SGMS2 gene.)
Rabbit SGMS2 Polyclonal Antibody | anti-SGMS2 antibody
SGMS2 Antibody (C-term)
Gene Names
SGMS2; SMS2
Reactivity
Human, mouse (Predicted Reactivity: Monkey, Rat)
Applications
ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SGMS2, Antibody; SGMS2 Antibody (C-term); Phosphatidylcholine:ceramide cholinephosphotransferase 2; Sphingomyelin synthase 2; SGMS2; SMS2; anti-SGMS2 antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Monkey, Rat)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
This SGMS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 338-365 amino acids from the C-terminal region of human SGMS2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
338-365
Sequence Length
365
Applicable Applications for anti-SGMS2 antibody
ELISA, WB (Western Blot)
Function
Sphingomyelin synthases synthesize the sphingolipid, sphingomyelin, through transfer of the phosphatidyl head group, phosphatidylcholine, on to the primary hydroxyl of ceramide (PubMed: ). The reaction is bidirectional depending on the respective levels of the sphingolipid and ceramide. Plasma membrane SMS2 can also convert phosphatidylethanolamine (PE) to ceramide phosphatidylethanolamine (CPE). Major form in liver. Required for cell growth in certain cell types. Regulator of cell surface levels of ceramide, an important mediator of signal transduction and apoptosis. Regulation of sphingomyelin (SM) levels at the cell surface affects insulin sensitivity. Involved in the regulation of bone mineralization (PubMed:30779713).
Cellular Location
Cell membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Note=Primarily localized at the plasma membrane with a small fraction at the Golgi apparatus.
Tissue Location
Brain, heart, kidney, liver, muscle and stomach. Also expressed in a number of cell lines such as carcinoma HeLa cells, hepatoma Hep-G2 cells, and colon carcinoma Caco-2 cells
Antigen Region
338-365
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of SGMS2 (arrow) using rabbit polyclonal SGMS2 Antibody (C-term)(AAA283932). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SGMS2 gene.)
WB (Western Blot)
(Western blot analysis of SGMS2 (arrow) using rabbit polyclonal SGMS2 Antibody (C-term)(AAA283932). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SGMS2 gene.)
WB (Western Blot)
(Western blot analysis of SGMS2 Antibody (C-term) (AAA283932) in mouse spleen tissue lysates (35ug/lane). SGMS2 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of SGMS2 Antibody (C-term) (AAA283932) in mouse spleen tissue lysates (35ug/lane). SGMS2 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of SGMS2 Antibody (C-term) (AAA283932) in HepG2 cell line lysates (35ug/lane). SGMS2 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of SGMS2 Antibody (C-term) (AAA283932) in HepG2 cell line lysates (35ug/lane). SGMS2 (arrow) was detected using the purified Pab.)
Related Product Information for anti-SGMS2 antibody
Sphingomyelin, a major component of cell and Golgi membranes, is made by the transfer of phosphocholine from phosphatidylcholine onto ceramide, with diacylglycerol as a side product. SGMS2 is an enzyme that catalyzes this reaction primarily at the cell membrane. The synthesis is reversible, and this enzyme can catalyze the reaction in either direction. This protein is required for cell growth.
Product Categories/Family for anti-SGMS2 antibody
References
Ternes, P., et al. J. Lipid Res. 50(11):2270-2277(2009)
Liu, J., et al. Arterioscler. Thromb. Vasc. Biol. 29(6):850-856(2009)
Tani, M., et al. Biochem. Biophys. Res. Commun. 381(3):328-332(2009)
Liu, J., et al. Arterioscler. Thromb. Vasc. Biol. 29(6):850-856(2009)
Tani, M., et al. Biochem. Biophys. Res. Commun. 381(3):328-332(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42280
NCBI Official Full Name
phosphatidylcholine:ceramide cholinephosphotransferase 2
NCBI Official Synonym Full Names
sphingomyelin synthase 2
NCBI Official Symbol
SGMS2
NCBI Official Synonym Symbols
SMS2
NCBI Protein Information
phosphatidylcholine:ceramide cholinephosphotransferase 2
UniProt Protein Name
Phosphatidylcholine:ceramide cholinephosphotransferase 2
UniProt Gene Name
SGMS2
UniProt Synonym Gene Names
SMS2
UniProt Entry Name
SMS2_HUMAN
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Product Notes
The SGMS2 sgms2 (Catalog #AAA283932) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 338-365. The SGMS2 Antibody (C-term) reacts with Human, mouse (Predicted Reactivity: Monkey, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's SGMS2 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the SGMS2 sgms2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SGMS2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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