FCM/FACS (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AP22321a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22321a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Rabbit anti-Human SH2B3 Polyclonal Antibody | anti-SH2B3 antibody
SH2B3 Antibody (N-Term)
Gene Names
SH2B3; LNK; IDDM20
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot
Synonyms
SH2B3, Antibody; SH2B3 Antibody (N-Term); Purified Rabbit Polyclonal Antibody (Pab); SH2B adapter protein 3; Lymphocyte adapter protein; Lymphocyte-specific adapter protein Lnk; Signal transduction protein Lnk; SH2B3; LNK; anti-SH2B3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Clone Number
RB57762
Specificity
This SH2B3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 67-101 amino acids from the human region of human SH2B3.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applicable Applications for anti-SH2B3 antibody
FCM/FACS (Flow Cytometry), WB (Western Blot)
Antigen Source
Human
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AP22321a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22321a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AP22321a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22321a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
WB (Western Blot)
(Anti-SH2B3 Antibody (N-Term) at 1:2000 dilution + U-251 MG whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 63 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-SH2B3 Antibody (N-Term) at 1:2000 dilution + U-251 MG whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 63 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-SH2B3 antibody
Links T-cell receptor activation signal to phospholipase C-gamma-1, GRB2 and phosphatidylinositol 3-kinase.
Product Categories/Family for anti-SH2B3 antibody
References
Li Y.,et al.J. Immunol. 164:5199-5206(2000).Bartholomew M.A.,et al.Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases.Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.Todd J.A.,et al.Nat. Genet. 39:857-864(2007).Hunt K.A.,et al.Nat. Genet. 40:395-402(2008).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
63,225 Da
NCBI Official Full Name
SH2B adapter protein 3 isoform 2
NCBI Official Synonym Full Names
SH2B adaptor protein 3
NCBI Official Symbol
SH2B3
NCBI Official Synonym Symbols
LNK; IDDM20
NCBI Protein Information
SH2B adapter protein 3; lymphocyte-specific adapter protein Lnk; signal transduction protein Lnk
UniProt Protein Name
SH2B adapter protein 3
UniProt Gene Name
SH2B3
UniProt Synonym Gene Names
LNK
UniProt Entry Name
SH2B3_HUMAN
Similar Products
Product Notes
The SH2B3 sh2b3 (Catalog #AAA290970) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SH2B3 Antibody (N-Term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SH2B3 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the SH2B3 sh2b3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SH2B3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.