IF (Immunofluorescence)
(AAA325149 staining RAW264.7 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Rabbit anti-Human, Mouse SHANK2 Polyclonal Antibody | anti-SHANK2 antibody
SHANK2 Antibody
Gene Names
SHANK2; SHANK; AUTS17; CORTBP1; CTTNBP1; ProSAP1; SPANK-3
Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
SHANK2, Antibody; SHANK2 Antibody; AUTS17; Cortactin binding protein 1; Cortactin SH3 domain-binding protein; Cortactin-binding protein 1; CortBP1; CTTNBP1; GKAP/SAPAP interacting protein; GKAP/SAPAP-interacting protein; KIAA1022; Proline rich synapse associated protein 1; Proline-rich synapse-associated protein 1; ProSAP1; SH3 and multiple ankyrin repeat domains 2; SH3 and multiple ankyrin repeat domains protein 2; SHAN2_RAT; SHANK; Shank2; SPANK-3; anti-SHANK2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
SHANK2 antibody detects endogenous levels of total SHANK2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1849
Applicable Applications for anti-SHANK2 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide
Subcellular Location
Apical Cell Membrane. Cytoplasm. Cell Junction, Synapse. Cell Junction, Synapse, Postsynaptic Cell Membrane, Postsynaptic Density. Cell Projection, Growth Cone. Cell Projection, Dendritic Spine. Colocalizes with cortactin in growth cones in differentiating hippocampal neurons. Present in the dendritic spines of cerebellar Purkinje cells (By similarity). Colocalizes with cortactin in growth cones in differentiating hippocampal neurons. Colocalized with PDE4D to the apical membrane of colonic crypt cells.
Tissue Specificity
Isoform 3 is present in epithelial colonic cells (at protein level).
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (100%), Bovine (91%), Horse (100%), Sheep (91%), Rabbit (100%), Dog (100%), Chicken (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA325149 staining RAW264.7 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IF (Immunofluorescence)
(AAA325149 staining RAW264.7 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from RAW264.7 cells using SHANK2 antibody.)
WB (Western Blot)
(Western blot analysis of extracts from RAW264.7 cells using SHANK2 antibody.)
Related Product Information for anti-SHANK2 antibody
Function: Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.
Subunit Structure: Is part of a complex with DLG4/PSD-95 and DLGAP1/GKAP. Interacts with CTTN/cortactin SH3 domain, DLGAP1/GKAP and alpha-latrotoxin receptor 1. Interacts with DNM2, DBNL, GRID2, BAIAP2, SLC9A3, PLCB3 and CFTR. Interacts (via proline-rich region) with PDE4D. Interacts with ABI1 (via SH3 domain).
Similarity: The PDZ domain is required for interaction with GRID2, PLCB3, CFTR and SLC9A3. Belongs to the SHANK family.
Subunit Structure: Is part of a complex with DLG4/PSD-95 and DLGAP1/GKAP. Interacts with CTTN/cortactin SH3 domain, DLGAP1/GKAP and alpha-latrotoxin receptor 1. Interacts with DNM2, DBNL, GRID2, BAIAP2, SLC9A3, PLCB3 and CFTR. Interacts (via proline-rich region) with PDE4D. Interacts with ABI1 (via SH3 domain).
Similarity: The PDZ domain is required for interaction with GRID2, PLCB3, CFTR and SLC9A3. Belongs to the SHANK family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 135 kD
Predicted: 159 kDa
Predicted: 159 kDa
NCBI Official Full Name
SH3 and multiple ankyrin repeat domains protein 2 isoform 1
NCBI Official Synonym Full Names
SH3 and multiple ankyrin repeat domains 2
NCBI Official Symbol
SHANK2
NCBI Official Synonym Symbols
SHANK; AUTS17; CORTBP1; CTTNBP1; ProSAP1; SPANK-3
NCBI Protein Information
SH3 and multiple ankyrin repeat domains protein 2
UniProt Protein Name
SH3 and multiple ankyrin repeat domains protein 2
UniProt Gene Name
SHANK2
UniProt Synonym Gene Names
CORTBP1; KIAA1022; PROSAP1; Shank2; CortBP1
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Product Notes
The SHANK2 shank2 (Catalog #AAA325149) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SHANK2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SHANK2 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the SHANK2 shank2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SHANK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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