Sheep anti Human IgG3 (subclass specific) Secondary Antibody
Sheep anti Human IgG3 (subclass specific)
Reactivity
Human
Synonyms
Sheep anti Human IgG3 (subclass specific), Secondary Antibody; Sheep anti Human IgG3 (subclass specific); Sheep anti Human IgG3 (subclass specific) secondary antibody
Host
Sheep
Reactivity
Human
Clonality
Polyclonal
Specificity
Precipitating polyclonal Sheep antisera to Human IgG3, subclass specific.
Source
Purified homogenous IgG3 is isolated from human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Product Type
Secondary Antibodies
Product
Delipidated, heat inactivated lyophilized stable whole serum. No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled sheep serum. No foreign proteins added. It is reconstituted by adding 1 ml sterile distilled water.
Reactivity Note
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
Preparation and Storage
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 degree C; prolonged storage at or below -20 degree C. Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Related Product Information for Sheep anti Human IgG3 (subclass specific) secondary antibody
The activity of the antiserum is directed to human IgG3 subclass. It does not react with any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In precipitating techniques as immunoelectrophoresis and double radial immunodiffusion to identify and measure the concentration of IgG3 subclass in serum or other body fluids. Human IgG constitutes about 75% of the total Immunoglobulins in the serum of normal adults. It consists of four subclasses which are distinguished in isotypic heterogeneity of the gamma chains, and in physicochemical, biological and effector properties. The term “subclass” designates the high degree of homology in amino acid sequences and antigenic structure. In normal adult serum, the approximate percentage composition of IgG with respect to its subclasses is: IgG1: 65%, IgG2: 23%, IgG3: 8% and IgG4: 4%. All four subclasses appear in both isotypes of the light chain. Normal serum thus contains eight different molecular forms of IgG, the absolute and relative distribution being genetically controlled and resulting from individual responses to the antigenicity of the environment. Antigenic determinants carrying isotypic subclass specificity can be situated on any of the three structurally very similar sub-regions o the gamma chains, known as domains. In IgG, some antigenic determinants are shared by two or three subclasses, but not by all four. Consequently, antibodies to such determinants will not be strictly subclass-specific. For the identification of human IgG3 in cells and tissues by immunofluorescence or immunocytochemical peroxidase techniques, or in body fluids by ELISA and Western blotting type procedures, AAA Biotech, Inc. monoclonal antibody reagents to the individual subclasses are available.
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Product Notes
The Sheep anti Human IgG3 (subclass specific) (Catalog #AAA77569) is a Secondary Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Sheep anti Human IgG3 (subclass specific) reacts with Human and may cross-react with other species as described in the data sheet. It is sometimes possible for the material contained within the vial of "Sheep anti Human IgG3 (subclass specific), Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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