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WB (Western Blot)
(Figure 4 Western Blot Validation in Rat TissuesLoading: 15 μg of lysates per lane.Antibodies: SIGLEC15, 9017 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
Rabbit SIGLEC15 Polyclonal Antibody | anti-SIGLEC15 antibody
SIGLEC15 Antibody
Gene Names
SIGLEC15; CD33L3; HsT1361; SIGLEC-15
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
SIGLEC15 Antibody is affinity chromatography purified via peptide column
Synonyms
SIGLEC15, Antibody; SIGLEC15 Antibody; sialic acid binding Ig-like lectin 15; SIGLEC15 Antibody: CD33L3; HsT1361; SIGLEC-15; CD33L3; Sialic acid-binding Ig-like lectin 15; CD33 antigen-like 3; Siglec-15; anti-SIGLEC15 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
SIGLEC15 Antibody is affinity chromatography purified via peptide column
Form/Format
Liquid
SIGLEC15 Antibody is supplied in PBS containing 0.02% sodium azide.
SIGLEC15 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-SIGLEC15 antibody
WB (Western Blot), ELISA
Source
Human
Immunogen
Anti-SIGLEC15 antibody (9017) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human SIGLEC15.
The immunogen is located within amino acids 270-320 of SIGLEC15.
The immunogen is located within amino acids 270-320 of SIGLEC15.
Isoforms
Human SIGLEC15 has 2 isoforms, including isoform 1 (328aa, 36kD) and isoform 2 (174aa, 18kD).
Mouse SIGLEC15 has one isoform (342aa, 37kD) and Rat SIGLEC15 also has one isoform (341aa, 37kD).
9017 can detect human and possibly mouse and rat isoforms.
Mouse SIGLEC15 has one isoform (342aa, 37kD) and Rat SIGLEC15 also has one isoform (341aa, 37kD).
9017 can detect human and possibly mouse and rat isoforms.
Conjugation
Unconjugated
Preparation and Storage
SIGLEC15 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
WB (Western Blot)
(Figure 4 Western Blot Validation in Rat TissuesLoading: 15 μg of lysates per lane.Antibodies: SIGLEC15, 9017 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Figure 4 Western Blot Validation in Rat TissuesLoading: 15 μg of lysates per lane.Antibodies: SIGLEC15, 9017 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Figure 3 Western Blot Validation in Mouse TissuesLoading: 15 μg of lysates per lane.Antibodies: SIGLEC15, 9017 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Figure 3 Western Blot Validation in Mouse TissuesLoading: 15 μg of lysates per lane.Antibodies: SIGLEC15, 9017 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Figure 2 Western Blot Validation in Human TissuesLoading: 15 μg of lysates per lane.Antibodies: SIGLEC15, 9017 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Figure 2 Western Blot Validation in Human TissuesLoading: 15 μg of lysates per lane.Antibodies: SIGLEC15, 9017 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Figure 1 Western Blot Validation in Human and Mouse Cell LinesLoading: 15 μg of lysates per lane.Antibodies: SIGLEC15, 9017 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Figure 1 Western Blot Validation in Human and Mouse Cell LinesLoading: 15 μg of lysates per lane.Antibodies: SIGLEC15, 9017 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
Related Product Information for anti-SIGLEC15 antibody
SIGLEC15 Antibody: Siglecs are vertebrate cell-surface lectins that recognize sialylated glycans and are involved in many physiological processes, such as glycoprotein turnover, cellular trafficking, and pathogen recognition. Most Siglecs are expressed on cells of the immune system. SIGLEC15 is a type-I transmembrane protein consisting of two immunoglobulin (Ig)-like domains, a transmembrane domain containing a lysine residue, and a short cytoplasmic tail. SIGLEC15 can interact with the activating adaptor molecules DAP12/10. Its activating signaling potential and unique preference for glycan recognition implies that SIGLEC15 may be involved in the immune surveillance of tumors and probably plays a conserved, regulatory role in the immune system of vertebrates.
Product Categories/Family for anti-SIGLEC15 antibody
References
Crocker and Redelinghuys. Biochem. Soc. Trans. 2008; 36:1467-71.
Angata et al. Glycobiology 2007; 17:838-46.
Hiruma et al. Biochem. Biophys. Res. Commun. 2011; 409:424-9.
Angata et al. Glycobiology 2007; 17:838-46.
Hiruma et al. Biochem. Biophys. Res. Commun. 2011; 409:424-9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,086 Da
NCBI Official Full Name
sialic acid-binding Ig-like lectin 15
NCBI Official Synonym Full Names
sialic acid binding Ig-like lectin 15
NCBI Official Symbol
SIGLEC15
NCBI Official Synonym Symbols
CD33L3; HsT1361; SIGLEC-15
NCBI Protein Information
sialic acid-binding Ig-like lectin 15; CD33 antigen-like 3; CD33 molecule-like 3
UniProt Protein Name
Sialic acid-binding Ig-like lectin 15
UniProt Gene Name
SIGLEC15
UniProt Synonym Gene Names
CD33L3; Siglec-15
UniProt Entry Name
SIG15_HUMAN
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Product Notes
The SIGLEC15 siglec15 (Catalog #AAA40920) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SIGLEC15 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SIGLEC15 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the SIGLEC15 siglec15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIGLEC15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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