IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Rabbit anti-Human, mouse SIGLEC9 Polyclonal Antibody | anti-SIGLEC9 antibody
SIGLEC9 Antibody (C-term)
Gene Names
SIGLEC9; CD329; CDw329; FOAP-9; siglec-9; OBBP-LIKE
Reactivity
Human, mouse
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SIGLEC9, Antibody; SIGLEC9 Antibody (C-term); Sialic acid-binding Ig-like lectin 9; Siglec-9; CDw329; Protein FOAP-9; CD329; SIGLEC9; anti-SIGLEC9 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This SIGLEC9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 385-415 amino acids from the C-terminal region of human SIGLEC9.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
385-415
Sequence Length
463
Applicable Applications for anti-SIGLEC9 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot)
(The anti-Siglec9 C-term Pab is used in Western blot to detect Siglec9 in mouse liver tissue lysate.)
WB (Western Blot)
(The anti-Siglec9 C-term Pab is used in Western blot to detect Siglec9 in mouse liver tissue lysate.)
WB (Western Blot)
(SIGLEC9 Antibody western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the SIGLEC9 antibody detected the SIGLEC9 protein (arrow).)
WB (Western Blot)
(SIGLEC9 Antibody western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the SIGLEC9 antibody detected the SIGLEC9 protein (arrow).)
Related Product Information for anti-SIGLEC9 antibody
SIGLEC9 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. This protein is expressed by peripheral blood leukocytes (neutrophils and monocytes but not eosinophils). It is found in liver, fetal liver, bone marrow, placenta, spleen and in lower levels in skeletal muscle, fetal brain, stomach, lung, thymus, prostate, brain, mammary, adrenal gland, colon, trachea, cerebellum, testis, small intestine and spinal cordon. SIGLEC9 contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
Product Categories/Family for anti-SIGLEC9 antibody
References
Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003). Zhang, J.Q., et al., J. Biol. Chem. 275(29):22121-22126 (2000). Foussias, G., et al., Genomics 67(2):171-178 (2000). Angata, T., et al., J. Biol. Chem. 275(29):22127-22135 (2000). Yousef, G.M., et al., Anticancer Res. 19 (4B), 2843-2852 (1999).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50082
NCBI Official Full Name
sialic acid-binding Ig-like lectin 9 isoform 2
NCBI Official Synonym Full Names
sialic acid binding Ig-like lectin 9
NCBI Official Symbol
SIGLEC9
NCBI Official Synonym Symbols
CD329; CDw329; FOAP-9; siglec-9; OBBP-LIKE
NCBI Protein Information
sialic acid-binding Ig-like lectin 9
UniProt Protein Name
Sialic acid-binding Ig-like lectin 9
UniProt Gene Name
SIGLEC9
UniProt Synonym Gene Names
Siglec-9
UniProt Entry Name
SIGL9_HUMAN
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Product Notes
The SIGLEC9 siglec9 (Catalog #AAA288044) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 385-415. The SIGLEC9 Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SIGLEC9 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the SIGLEC9 siglec9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIGLEC9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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