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product-image-AAA142316_AD8.jpg Application Data (Knockout Validation: Lane 1: Wild-type K562 cell lysate; Lane 2: STAT5B knockout K562 cell lysate; Predicted MW: 90kd Observed MW: 95kd Primary Ab: 3ug/ml Rabbit Anti-Human STAT5B Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

Rabbit anti-Human Signal Transducer And Activator Of Transcription 5B Polyclonal Antibody | anti-STAT5B antibody

Polyclonal Antibody to Signal Transducer And Activator Of Transcription 5B (STAT5B)

Gene Names
STAT5B; STAT5
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Affinity Chromatography
Synonyms
Signal Transducer And Activator Of Transcription 5B, Antibody; Polyclonal Antibody to Signal Transducer And Activator Of Transcription 5B (STAT5B); anti-STAT5B antibody
Ordering
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against STAT5B. It has beenselected for its ability to recognize STAT5B in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- AVPD KVLWPQLCEA LNMKFKAEVQ SNRGLTKENL VFLAQKLFNN SSSHLEDYSG LSVSWSQFNR ENLPGRNYTF WQWFDGVMEV LKKHLKPHWN DGAILGFVNK QQAHDLLINK PDGTFLLRFS DSEIGGITIA WKFDSQERMF WNLMPFTTRD FSIRSLADRL GDLNYLIYVF PDRPKDEVYS KYYTPVPCES ATAKAVDGYV KPQIKQVVPE FVNASADAGG GSATYMDQAP SPAVCPQAHY NMYPQNPDS
Sequence Length
787
Applicable Applications for anti-STAT5B antibody
ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot)
Fragment
STAT5B (Ala497~Ser749)
Organism Species
Homo sapiens (Human)
Cross Reactivity
Human
Conjugate
No Conjugate
Immunogen
Recombinant STAT5B (Ala497~Ser749) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Application Data

(Knockout Validation: Lane 1: Wild-type K562 cell lysate; Lane 2: STAT5B knockout K562 cell lysate; Predicted MW: 90kd Observed MW: 95kd Primary Ab: 3ug/ml Rabbit Anti-Human STAT5B Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

product-image-AAA142316_AD8.jpg Application Data (Knockout Validation: Lane 1: Wild-type K562 cell lysate; Lane 2: STAT5B knockout K562 cell lysate; Predicted MW: 90kd Observed MW: 95kd Primary Ab: 3ug/ml Rabbit Anti-Human STAT5B Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry)

(DAB staining on IHC-P; Samples: Human Kidney Tissue.)

product-image-AAA142316_IHC10.jpg IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Kidney Tissue.)

IHC (Immunohistochemisry)

(DAB staining on IHC-P; Samples: Human Brain Tissue)

product-image-AAA142316_IHC11.jpg IHC (Immunohistochemisry) (DAB staining on IHC-P; Samples: Human Brain Tissue)

WB (Western Blot)

(Western Blot: Sample: Recombinant STAT5B, Human.)

product-image-AAA142316_WB13.jpg WB (Western Blot) (Western Blot: Sample: Recombinant STAT5B, Human.)

WB (Western Blot)

(Western Blot: Sample: Human Hela Cells.)

product-image-AAA142316_WB15.jpg WB (Western Blot) (Western Blot: Sample: Human Hela Cells.)
Product Categories/Family for anti-STAT5B antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
89,866 Da
NCBI Official Full Name
signal transducer and activator of transcription 5B
NCBI Official Synonym Full Names
signal transducer and activator of transcription 5B
NCBI Official Symbol
STAT5B
NCBI Official Synonym Symbols
STAT5
NCBI Protein Information
signal transducer and activator of transcription 5B
UniProt Protein Name
Signal transducer and activator of transcription 5B
UniProt Gene Name
STAT5B

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Product Notes

The STAT5B stat5b (Catalog #AAA142316) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Signal Transducer And Activator Of Transcription 5B (STAT5B) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Signal Transducer And Activator Of Transcription 5B can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the STAT5B stat5b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- AVPD KVLWPQLCEA LNMKFKAEVQ SNRGLTKENL VFLAQKLFNN SSSHLEDYSG LSVSWSQFNR ENLPGRNYTF WQWFDGVMEV LKKHLKPHWN DGAILGFVNK QQAHDLLINK PDGTFLLRFS DSEIGGITIA WKFDSQERMF WNLMPFTTRD FSIRSLADRL GDLNYLIYVF PDRPKDEVYS KYYTPVPCES ATAKAVDGYV KPQIKQVVPE FVNASADAGG GSATYMDQAP SPAVCPQAHY NMYPQNPDS. It is sometimes possible for the material contained within the vial of "Signal Transducer And Activator Of Transcription 5B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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