WB (Western Blot)
(Western blot analysis of various lysates, using SIM2 Rabbit pAb (AAA281250) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
Rabbit SIM2 Polyclonal Antibody | anti-SIM2 antibody
SIM2 Polyclonal Antibody
Gene Names
SIM2; SIM; bHLHe15; HMC13F06; HMC29C01
Applications
Western Blot
Purity
Affinity Purification
Synonyms
SIM2, Antibody; SIM2 Polyclonal Antibody; bHLHe15; HMC13F06; HMC29C01; SIM; anti-SIM2 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Sequence
LSLEQVSTAKSQDSWRTALSTSQETRKLVKPKNTKMKTKLRTNPYPPQQYSSFQMDKLECGQLGNWRASPPASAAAPPELQPHSESSDLLYTPSYSLPFSYHYGHFPLDSHVFSSKKPMLPAKFGQPQGSPCEVARFFLSTLPASGECQWHYANPLVPSSSSPAKNPPEPPANTARHSLVPSYE
Sequence Length
667
Applicable Applications for anti-SIM2 antibody
WB (Western Blot)
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 342-525 of human SIM2 (NP_033664.2).
Species Reactivity / Cross Reactivity
Human, Mouse, Rat
Preparation and Storage
Store at -20°C. Avoid freeze / thaw cycles.
WB (Western Blot)
(Western blot analysis of various lysates, using SIM2 Rabbit pAb (AAA281250) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
WB (Western Blot)
(Western blot analysis of various lysates, using SIM2 Rabbit pAb (AAA281250) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
Related Product Information for anti-SIM2 antibody
This gene represents a homolog of the Drosophila single-minded (sim) gene, which encodes a transcription factor that is a master regulator of neurogenesis. The encoded protein is ubiquitinated by RING-IBR-RING-type E3 ubiquitin ligases, including the parkin RBR E3 ubiquitin protein ligase. This gene maps within the so-called Down syndrome chromosomal region, and is thus thought to contribute to some specific Down syndrome phenotypes. Alternative splicing of this gene results in multiple transcript variants.
Product Categories/Family for anti-SIM2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed MW: 73kDa
Calculated MW: 73kDa
Calculated MW: 73kDa
NCBI Official Full Name
single-minded homolog 2 long isoform
NCBI Official Synonym Full Names
single-minded family bHLH transcription factor 2
NCBI Official Symbol
SIM2
NCBI Official Synonym Symbols
SIM; bHLHe15; HMC13F06; HMC29C01
NCBI Protein Information
single-minded homolog 2
UniProt Protein Name
Single-minded homolog 2
UniProt Gene Name
SIM2
UniProt Synonym Gene Names
BHLHE15; bHLHe15
Similar Products
Product Notes
The SIM2 sim2 (Catalog #AAA281250) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's SIM2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the SIM2 sim2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: LSLEQVSTAK SQDSWRTALS TSQETRKLVK PKNTKMKTKL RTNPYPPQQY SSFQMDKLEC GQLGNWRASP PASAAAPPEL QPHSESSDLL YTPSYSLPFS YHYGHFPLDS HVFSSKKPML PAKFGQPQGS PCEVARFFLS TLPASGECQW HYANPLVPSS SSPAKNPPEP PANTARHSLV PSYE. It is sometimes possible for the material contained within the vial of "SIM2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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