WB (Western Blot)
(Detection of human Sin1 by western blot and immunoprecipitation. Samples: Whole cell lysate (50 and 15 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Sin1 antibody AAA211214 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). Detection: Chemiluminescence with exposure times of 7 minutes (A) and 30 seconds (B).)
Rabbit anti-Human Sin1 Polyclonal Antibody | anti-Sin1 antibody
Rabbit anti-Sin1 Antibody, Affinity Purified
Gene Names
MAPKAP1; MIP1; SIN1; JC310; SIN1b; SIN1g
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Sin1, Antibody; Rabbit anti-Sin1 Antibody, Affinity Purified; SIN1b; ras inhibitor MGC2745; TORC2 subunit MAPKAP1; target of rapamycin complex 2 subunit MAPKAP1; stress-activated protein kinase-interacting 1; Stress-activated map kinase-interacting protein 1; stress-activated map kinase interacting protein 1; SIN1g; SAPK-interacting protein 1; JC310; mSIN1; mitogen-activated protein kinase 2-associated protein 1; MIP1; MEKK2-interacting protein 1; SIN1; Target of rapamycin complex 2 subunit MAPKAP1; mitogen-activated protein kinase associated protein 1; anti-Sin1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-Sin1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse, Rat, Chicken, Sheep, Bovine, Orangutan
Immunogen
Between 472 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Sin1 by western blot and immunoprecipitation. Samples: Whole cell lysate (50 and 15 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Sin1 antibody AAA211214 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). Detection: Chemiluminescence with exposure times of 7 minutes (A) and 30 seconds (B).)
WB (Western Blot)
(Detection of human Sin1 by western blot and immunoprecipitation. Samples: Whole cell lysate (50 and 15 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Sin1 antibody AAA211214 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). Detection: Chemiluminescence with exposure times of 7 minutes (A) and 30 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human Sin1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Sin1 (Cat. No. AAA211214 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human Sin1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Sin1 (Cat. No. AAA211214 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-Sin1 antibody
Stress-activated map kinase-interacting protein 1 (Sin1) is highly similar to the yeast stress- activated protein kinase SIN1. Sin1 is an essential mTORC2 subunit that is required to act as the PDK2 that phosphorylates Akt/PKB at Ser 473. Sin1 has been implicated as a scaffolding protein. Sin1 is required for rictor binding in the mTORC2 complex. Sin1 has also been shown to be important to the SAPK (stress-activated kinase) signaling pathway and in the nucleus may act as a scaffold between ATF-2 and p38 to facilitate p38-induced phosphorylation of ATF-2. Sin1 is also found to form a complex with c-Jun N-terminal kinase (JNK) and may similarly function as a scaffolding protein in JNK signaling.
Product Categories/Family for anti-Sin1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
41,838 Da
NCBI Official Full Name
target of rapamycin complex 2 subunit MAPKAP1 isoform 1
NCBI Official Synonym Full Names
mitogen-activated protein kinase associated protein 1
NCBI Official Symbol
MAPKAP1
NCBI Official Synonym Symbols
MIP1; SIN1; JC310; SIN1b; SIN1g
NCBI Protein Information
target of rapamycin complex 2 subunit MAPKAP1; MEKK2-interacting protein 1; SAPK-interacting protein 1; TORC2 subunit MAPKAP1; mSIN1; mitogen-activated protein kinase 2-associated protein 1; ras inhibitor MGC2745; stress-activated map kinase interacting p
UniProt Protein Name
Target of rapamycin complex 2 subunit MAPKAP1
UniProt Gene Name
MAPKAP1
UniProt Synonym Gene Names
MIP1; SIN1; TORC2 subunit MAPKAP1; SAPK-interacting protein 1; mSIN1
UniProt Entry Name
SIN1_HUMAN
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Product Notes
The Sin1 mapkap1 (Catalog #AAA211214) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Sin1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Sin1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the Sin1 mapkap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sin1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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