WB (Western Blot)
(SIRT2 antibody (pAb) tested by Western blot. Cytoplasmic extract of K-562 cells (25 ug per lane) probed with SIRT2 antibody (1:500).)
Rabbit anti-Human SIRT2 Polyclonal Antibody | anti-SIRT2 antibody
SIRT2 antibody (pAb)
Gene Names
SIRT2; SIR2; SIR2L; SIR2L2
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Affinity Purified
Synonyms
SIRT2, Antibody; SIRT2 antibody (pAb); SIR2; SIR2L; SIR2L2; anti-SIRT2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-SIRT2 antibody
WB (Western Blot), IP (Immunoprecipitation)
Immunogen
This SIRT2 antibody was raised against a synthetic peptide derived from the C-terminal region of human SIRT2.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
WB (Western Blot)
(SIRT2 antibody (pAb) tested by Western blot. Cytoplasmic extract of K-562 cells (25 ug per lane) probed with SIRT2 antibody (1:500).)
WB (Western Blot)
(SIRT2 antibody (pAb) tested by Western blot. Cytoplasmic extract of K-562 cells (25 ug per lane) probed with SIRT2 antibody (1:500).)
WB (Western Blot)
(SIRT2 antibody (pAb) tested by Immunoprecipitation. 10 ul of SIRT2 antibody was used to immunoprecipitate SIRT2 from 250 ug of K-562 cytoplasmic cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the SIRT2 antibody at a dilution of 1:500.)
WB (Western Blot)
(SIRT2 antibody (pAb) tested by Immunoprecipitation. 10 ul of SIRT2 antibody was used to immunoprecipitate SIRT2 from 250 ug of K-562 cytoplasmic cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the SIRT2 antibody at a dilution of 1:500.)
Related Product Information for anti-SIRT2 antibody
Short Description: Our SIRT2 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Immunoprecipitation and Western blot; it has been shown to react with Human samples.
Background: SIRT2 is a member of the SIR2/Sirtuin family of NAD-dependant deacetylase enzymes. It has multiple targets for deacetylation, including tubulin, P53, HOXA10, FOXO3A, P300, FOXO1. SIRT2 suppresses adipogenesis by deacetylating FOXO1, promoting the binding of FOXO1 to PPARg and subsequent repression of PPARg mediated transcriptional activation. Similar to SIRT1, SIRT2 also deacetylates H4AcK16 similar to SIRT1, SIRT2 deacetylates histone H4 at lysine 16. SIRT2 is present in the cytoplasm during most of the cell cycle except in the G2/M transition and in mitosis, where it is found in the nucleus and in contact with chromatin and required for exit from mitosis.
Background: SIRT2 is a member of the SIR2/Sirtuin family of NAD-dependant deacetylase enzymes. It has multiple targets for deacetylation, including tubulin, P53, HOXA10, FOXO3A, P300, FOXO1. SIRT2 suppresses adipogenesis by deacetylating FOXO1, promoting the binding of FOXO1 to PPARg and subsequent repression of PPARg mediated transcriptional activation. Similar to SIRT1, SIRT2 also deacetylates H4AcK16 similar to SIRT1, SIRT2 deacetylates histone H4 at lysine 16. SIRT2 is present in the cytoplasm during most of the cell cycle except in the G2/M transition and in mitosis, where it is found in the nucleus and in contact with chromatin and required for exit from mitosis.
Product Categories/Family for anti-SIRT2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 43 kDa
Observed: 52 kDa
Observed: 52 kDa
NCBI Official Full Name
NAD-dependent protein deacetylase sirtuin-2 isoform 1
NCBI Official Synonym Full Names
sirtuin 2
NCBI Official Symbol
SIRT2
NCBI Official Synonym Symbols
SIR2; SIR2L; SIR2L2
NCBI Protein Information
NAD-dependent protein deacetylase sirtuin-2; sirtuin type 2; SIR2-like protein 2; sir2-related protein type 2; silent information regulator 2; regulatory protein SIR2 homolog 2; NAD-dependent deacetylase sirtuin-2
UniProt Protein Name
NAD-dependent protein deacetylase sirtuin-2
UniProt Gene Name
SIRT2
UniProt Synonym Gene Names
SIR2L; SIR2L2
UniProt Entry Name
SIR2_HUMAN
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Product Notes
The SIRT2 sirt2 (Catalog #AAA59944) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SIRT2 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SIRT2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the SIRT2 sirt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIRT2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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