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WB (Western Blot)
(Western Blot; Samples: Lane1: Jurkat cell lysate; Lane2: Raji cell lysate; Lane3: NIH/3T3 cell lysate; Lane4: RAW264.7 cell lysate;Primary Ab: 0.1ug/ml Rabbit Anti-Human SSB AntibodySecond Ab: 0.2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Rabbit anti-Human Sjogren Syndrome Antigen B (SSB) Polyclonal Antibody | anti-SSB antibody
Polyclonal Antibody to Sjogren Syndrome Antigen B (SSB)
Gene Names
SSB; La; LARP3
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Sjogren Syndrome Antigen B (SSB); Polyclonal Antibody; Polyclonal Antibody to Sjogren Syndrome Antigen B (SSB); Sjogren Syndrome Antigen B; LARP3; Lupus La Protein; Autoantigen La; La Ribonucleoprotein Domain Family; Member 3; Autoantigen SSB/La; anti-SSB antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against SSB. It has been selected for its ability to recognize SSB in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Liquid; PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Asn4~Glu194
Applicable Applications for anti-SSB antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 0.2-2ug/mL; 1:250-2500
IHC: 5-20ug/mL; 1:25-100
ICC: 5-20ug/mL; 1:25-100
IHC: 5-20ug/mL; 1:25-100
ICC: 5-20ug/mL; 1:25-100
Conjugation
Unconjugated
Research Area
Immune molecule
Preparation and Storage
Store at 4 degree C for frequent use. Store at -20 degree C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
WB (Western Blot)
(Western Blot; Samples: Lane1: Jurkat cell lysate; Lane2: Raji cell lysate; Lane3: NIH/3T3 cell lysate; Lane4: RAW264.7 cell lysate;Primary Ab: 0.1ug/ml Rabbit Anti-Human SSB AntibodySecond Ab: 0.2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot; Samples: Lane1: Jurkat cell lysate; Lane2: Raji cell lysate; Lane3: NIH/3T3 cell lysate; Lane4: RAW264.7 cell lysate;Primary Ab: 0.1ug/ml Rabbit Anti-Human SSB AntibodySecond Ab: 0.2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot; Samples: Lane1: Hela cell lysate; Lane2: 293T cell lysate; Lane3: Ramos cell lysate; Lane4: K562 cell lysate; Lane5: MCF7 cell lysate;Primary Ab: 0.1ug/ml Rabbit Anti-Human SSB AntibodySecond Ab: 0.2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot; Samples: Lane1: Hela cell lysate; Lane2: 293T cell lysate; Lane3: Ramos cell lysate; Lane4: K562 cell lysate; Lane5: MCF7 cell lysate;Primary Ab: 0.1ug/ml Rabbit Anti-Human SSB AntibodySecond Ab: 0.2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry-Paraffin)
(DAB staining on IHC-P;Sample: Porcine Lymph node TissuePrimary Ab: 10ug/ml Rabbit Anti-Human SSB AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry-Paraffin)
(DAB staining on IHC-P;Sample: Porcine Lymph node TissuePrimary Ab: 10ug/ml Rabbit Anti-Human SSB AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry-Paraffin)
(DAB staining on IHC-P;Sample: Porcine Small intestine TissuePrimary Ab: 10ug/ml Rabbit Anti-Human SSB AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry-Paraffin)
(DAB staining on IHC-P;Sample: Porcine Small intestine TissuePrimary Ab: 10ug/ml Rabbit Anti-Human SSB AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry-Paraffin)
(DAB staining on IHC-P;Sample: Porcine Cerebrum TissuePrimary Ab: 10ug/ml Rabbit Anti-Human SSB AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry-Paraffin)
(DAB staining on IHC-P;Sample: Porcine Cerebrum TissuePrimary Ab: 10ug/ml Rabbit Anti-Human SSB AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry-Paraffin)
(DAB staining on IHC-P;Sample: Porcine Colon TissuePrimary Ab: 10ug/ml Rabbit Anti-Human SSB AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry-Paraffin)
(DAB staining on IHC-P;Sample: Porcine Colon TissuePrimary Ab: 10ug/ml Rabbit Anti-Human SSB AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Product Categories/Family for anti-SSB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,837 Da
NCBI Official Full Name
lupus La protein
NCBI Official Synonym Full Names
Sjogren syndrome antigen B (autoantigen La)
NCBI Official Symbol
SSB
NCBI Official Synonym Symbols
La; LARP3
NCBI Protein Information
lupus La protein; SS-B; autoantigen La; la autoantigen; SS-B/La protein; lupus La antigen; sjoegren syndrome type B antigen; La ribonucleoprotein domain family, member 3
UniProt Protein Name
Lupus La protein
UniProt Gene Name
SSB
UniProt Synonym Gene Names
SS-B
UniProt Entry Name
LA_HUMAN
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Product Notes
The SSB ssb (Catalog #AAA21267) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Sjogren Syndrome Antigen B (SSB) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Sjogren Syndrome Antigen B (SSB) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.2-2ug/mL; 1:250-2500 IHC: 5-20ug/mL; 1:25-100 ICC: 5-20ug/mL; 1:25-100. Researchers should empirically determine the suitability of the SSB ssb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Asn4~Glu19 4. It is sometimes possible for the material contained within the vial of "Sjogren Syndrome Antigen B (SSB), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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