Rabbit anti-Human SLBP Polyclonal Antibody | anti-SLBP antibody

Rabbit anti-SLBP Antibody, Affinity Purified

Cat. Num. AAA212415

• Gene Names: SLBP; HBP
• Reactivity: Human
• Applications: Immunoprecipitation, Western Blot
• Purity: Antigen Affinity Purified
• Synonyms: SLBP, Antibody; Rabbit anti-SLBP Antibody, Affinity Purified; histone binding protein; stem-loop (histone) binding protein; Histone stem-loop-binding protein; histone RNA hairpin-binding protein; HBP; hairpin binding protein, histone; histone stem-loop binding protein; Histone RNA hairpin-binding protein; stem-loop binding protein; anti-SLBP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000ug/ml (varies by lot)

• Applicable Applications for anti-SLBP antibody: IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: Between 220 and C-terminus
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human SLBP by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-SLBP antibody AAA212415 (lot AAA212415-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

IP (Immunoprecipitation)

(Detection of human SLBP by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-SLBP antibody AAA212415 (lot AAA212415-1) used for IP at 6 ug/mg lysate. SLBP was also immunoprecipitated by rabbit anti-SLBP antibody BL14232. For blotting immunoprecipitated SLBP, AAA212415 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

Related Product Information for anti-SLBP antibody

Stem-loop-binding protein (SLBP) is an RNA-binding protein involved in the histone pre-mRNA processing. SLBP binds the stem-loop structure of replication-dependent histone pre-mRNAs and contributes to efficient 3'-end processing by stabilizing the complex between histone pre-mRNA and U7 small nuclear ribonucleoprotein (snRNP), via the histone downstream element (HDE). SLBP plays an important role in targeting mature histone mRNA from the nucleus to the cytoplasm and to the translation machinery. It stabilizes mature histone mRNA and could be involved in cell-cycle regulation of histone gene expression. It is also involved in the mechanism by which growing oocytes accumulate histone proteins that support early embryogenesis. SLBP binds to the 5' side of the stem-loop structure of histone pre-mRNAs [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q14493].

Product Categories/Family for anti-SLBP antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 5729862
• NCBI GeneID: 7884
• NCBI Accession #: NP_006518.1
• NCBI GenBank Nucleotide #: NM_006527.2
• UniProt Accession #: Q14493; B3KRJ5
• Molecular Weight: 31,286 Da
• NCBI Official Full Name: histone RNA hairpin-binding protein
• NCBI Official Synonym Full Names: stem-loop binding protein
• NCBI Official Symbol: SLBP
• NCBI Official Synonym Symbols: HBP
• NCBI Protein Information: histone RNA hairpin-binding protein; histone binding protein; hairpin binding protein, histone; histone stem-loop binding protein; histone stem-loop-binding protein; stem-loop (histone) binding protein
• UniProt Protein Name: Histone RNA hairpin-binding protein
• UniProt Gene Name: SLBP
• UniProt Synonym Gene Names: HBP
• UniProt Entry Name: SLBP_HUMAN