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IF (Immunofluorescence)
(AAA329142 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA329142 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Rabbit SLC16A8 Polyclonal Antibody | anti-SLC16A8 antibody
SLC16A8 Antibody
Gene Names
SLC16A8; MCT3; REMP
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
SLC16A8, Antibody; SLC16A8 Antibody; MCT 3; MCT3; Monocarboxylate transporter 3; Monocarboxylic acid transporter 3; OTTHUMP00000028510; REMP; Solute carrier 16 (monocarboxylic acid transporters) member 8; Solute carrier family 16 member 8; solute carrier family 16; member 8 (monocarboxylic acid transporter 3); anti-SLC16A8 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%)
Predicted Reactivity: Pig(100%)
Clonality
Polyclonal
Isotype
IgG
Specificity
SLC16A8 Antibody detects endogenous levels of total SLC16A8.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
504
Applicable Applications for anti-SLC16A8 antibody
ELISA
Immunogen
A synthesized peptide derived from human SLC16A8, corresponding to a region within C-terminal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA329142 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA329142 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
IF (Immunofluorescence)
(AAA329142 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA329142 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
IHC (Immunohistochemisry)
(AAA329142 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(AAA329142 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(AAA329142 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(AAA329142 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from Mouse testis, using SLC16A8 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Mouse testis, using SLC16A8 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-SLC16A8 antibody
Function: Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate (By similarity).
Subcellular Location: Cell membrane>Multi-pass membrane protein.
Tissue Specificity: Retinal pigment epithelium.
Similarity: Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.
Subcellular Location: Cell membrane>Multi-pass membrane protein.
Tissue Specificity: Retinal pigment epithelium.
Similarity: Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)52kD.
Predicted Molecular Weight: (Calculated)52kDa.
Predicted Molecular Weight: (Calculated)52kDa.
NCBI Official Full Name
monocarboxylate transporter 3
NCBI Official Synonym Full Names
solute carrier family 16 member 8
NCBI Official Symbol
SLC16A8
NCBI Official Synonym Symbols
MCT3; REMP
NCBI Protein Information
monocarboxylate transporter 3
UniProt Protein Name
Monocarboxylate transporter 3
UniProt Gene Name
SLC16A8
UniProt Synonym Gene Names
MCT3; MCT 3
UniProt Entry Name
MOT3_HUMAN
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Product Notes
The SLC16A8 slc16a8 (Catalog #AAA329142) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SLC16A8 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig(100%) and may cross-react with other species as described in the data sheet. AAA Biotech's SLC16A8 can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the SLC16A8 slc16a8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLC16A8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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