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FCM/FACS (Flow Cytometry)
(SLC25A37 Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human, rat SLC25A37 Polyclonal Antibody | anti-SLC25A37 antibody
SLC25A37 Antibody (C-term)
Gene Names
SLC25A37; MSC; MFRN; MSCP; HT015; MFRN1; PRO1278; PRO1584; PRO2217
Reactivity
Human, rat
Applications
Immunofluorescence, Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
SLC25A37, Antibody; SLC25A37 Antibody (C-term); Mitoferrin-1; Mitochondrial iron transporter 1; Mitochondrial solute carrier protein; Solute carrier family 25 member 37; SLC25A37; MFRN; MSCP; anti-SLC25A37 antibody
Host
Rabbit
Reactivity
Human, rat
Clonality
Polyclonal
Isotype
Ig
Specificity
This SLC25A37 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309-338 amino acids from the C-terminal region of human SLC25A37.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Positions
309-338
Sequence Length
338
Applicable Applications for anti-SLC25A37 antibody
IF (Immunofluorescence), WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA
Function
Mitochondrial iron transporter that specifically mediates iron uptake in developing erythroid cells, thereby playing an essential role in heme biosynthesis. The iron delivered into the mitochondria, presumably as Fe(2+), is then probably delivered to ferrochelatase to catalyze Fe(2+) incorporation into protoprophyrin IX to make heme (By similarity).
Cellular Location
Mitochondrion inner membrane; Multi-pass membrane protein.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(SLC25A37 Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(SLC25A37 Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemisry)
(SLC25A37 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SLC25A37 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(SLC25A37 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SLC25A37 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(SLC25A37 Antibody (C-term) western blot analysis in K562 cell line and rat spleen tissue lysates (35ug/lane).This demonstrates the SLC25A37 antibody detected the SLC25A37 protein (arrow).)
WB (Western Blot)
(SLC25A37 Antibody (C-term) western blot analysis in K562 cell line and rat spleen tissue lysates (35ug/lane).This demonstrates the SLC25A37 antibody detected the SLC25A37 protein (arrow).)
IF (Immunofluorescence)
(Immunofluorescent analysis of Hela cells, using SLC25A37 Antibody (C-term). AAA287998 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).)
IF (Immunofluorescence)
(Immunofluorescent analysis of Hela cells, using SLC25A37 Antibody (C-term). AAA287998 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).)
Related Product Information for anti-SLC25A37 antibody
SLC25A37 belongs to the SLC25 family of mitochondrial carrier proteins (Haitina et al., 2006 [PubMed 16949250]).[supplied by OMIM].
Product Categories/Family for anti-SLC25A37 antibody
References
Kaneko, K., et al. FEBS J. 276(5):1370-1382(2009) Haitina, T., et al. Genomics 88(6):779-790(2006) Shaw, G.C., et al. Nature 440(7080):96-100(2006) Li, Q.Z., et al. Mamm. Genome 12(11):830-836(2001)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 37323
NCBI Official Full Name
mitoferrin-1
NCBI Official Synonym Full Names
solute carrier family 25 (mitochondrial iron transporter), member 37
NCBI Official Symbol
SLC25A37
NCBI Official Synonym Symbols
MSC; MFRN; MSCP; HT015; MFRN1; PRO1278; PRO1584; PRO2217
NCBI Protein Information
mitoferrin-1
UniProt Protein Name
Mitoferrin-1
UniProt Gene Name
SLC25A37
UniProt Synonym Gene Names
MFRN; MSCP
UniProt Entry Name
MFRN1_HUMAN
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Product Notes
The SLC25A37 slc25a37 (Catalog #AAA287998) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 309-338. The SLC25A37 Antibody (C-term) reacts with Human, rat and may cross-react with other species as described in the data sheet. AAA Biotech's SLC25A37 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the SLC25A37 slc25a37 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLC25A37, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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