FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U2OS cells using anti-SLC3A1 antibody (AAA126240).Overlay histogram showing U2OS cells stained with AAA126240 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC3A1 Antibody (AAA126240, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rabbit SLC3A1 Polyclonal Antibody | anti-SLC3A1 antibody
Anti-SLC3A1 Antibody Picoband
Gene Names
SLC3A1; D2H; ATR1; NBAT; RBAT; CSNU1
Reactivity
Human, Monkey, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Functional Assay, ELISA
Purity
Immunogen affinity purified.
Synonyms
SLC3A1, Antibody; Anti-SLC3A1 Antibody Picoband; anti-SLC3A1 antibody
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (varies by lot)
Applicable Applications for anti-SLC3A1 antibody
WB (Western Blot), FCM/FACS (Flow Cytometry), ELISA
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Immunogen
E Coli-derived human SLC3A1 recombinant protein (Position: K70-R665).
Preparation and Storage
Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U2OS cells using anti-SLC3A1 antibody (AAA126240).Overlay histogram showing U2OS cells stained with AAA126240 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC3A1 Antibody (AAA126240, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U2OS cells using anti-SLC3A1 antibody (AAA126240).Overlay histogram showing U2OS cells stained with AAA126240 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC3A1 Antibody (AAA126240, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot)
(Figure 1. Western blot analysis of SLC3A1 using anti-SLC3A1 antibody (AAA126240).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: monkey COS-7 whole cell lysates,Lane 2: human Caco-2 whole cell lysates,Lane 3: rat NRK whole cell lysates,Lane 4: mouse HBZY whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC3A1 antigen affinity purified polyclonal antibody (#AAA126240) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SLC3A1 at approximately 110 kDa. The expected band size for SLC3A1 is at 79 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of SLC3A1 using anti-SLC3A1 antibody (AAA126240).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: monkey COS-7 whole cell lysates,Lane 2: human Caco-2 whole cell lysates,Lane 3: rat NRK whole cell lysates,Lane 4: mouse HBZY whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC3A1 antigen affinity purified polyclonal antibody (#AAA126240) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SLC3A1 at approximately 110 kDa. The expected band size for SLC3A1 is at 79 kDa.)
Related Product Information for anti-SLC3A1 antibody
Neutral and basic amino acid transport protein rBAT is a protein that in humans is encoded by the SLC3A1 gene. This gene encodes a type II membrane glycoprotein which is one of the components of the renal amino acid transporter which transports neutral and basic amino acids in the renal tubule and intestinal tract. Mutations and deletions in this gene are associated with cystinuria. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
Product Categories/Family for anti-SLC3A1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
78,852 Da
NCBI Official Full Name
SLC3A1 variant G
NCBI Official Synonym Full Names
solute carrier family 3 (amino acid transporter heavy chain), member 1
NCBI Official Symbol
SLC3A1
NCBI Official Synonym Symbols
D2H; ATR1; NBAT; RBAT; CSNU1
NCBI Protein Information
neutral and basic amino acid transport protein rBAT; SLC3A1 variant B; SLC3A1 variant C; SLC3A1 variant D; SLC3A1 variant E; SLC3A1 variant F; SLC3A1 variant G; amino acid transporter 1; solute carrier family 3 member 1; B(0,+)-type amino acid transport p
UniProt Protein Name
Neutral and basic amino acid transport protein rBAT
UniProt Gene Name
SLC3A1
UniProt Synonym Gene Names
RBAT; NBAT
UniProt Entry Name
SLC31_HUMAN
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Product Notes
The SLC3A1 slc3a1 (Catalog #AAA126240) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SLC3A1 Antibody Picoband reacts with Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SLC3A1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the SLC3A1 slc3a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLC3A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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