IF (Immunofluorescence)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degrees C. Samples were then incubated with primary Ab(AAA328178 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37 degrees C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue).)
Rabbit SLC4A7 Polyclonal Antibody | anti-SLC4A7 antibody
SLC4A7 Antibody
Gene Names
SLC4A7; NBC2; NBC3; SBC2; NBCN1; SLC4A6
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
SLC4A7, Antibody; SLC4A7 Antibody; Bicarbonate transporter; BT; NBC2; NBC3; S4A7; SBC2; SLC4A6; Sodium bicarbonate cotransporter 2; Sodium bicarbonate cotransporter 2b; Sodium bicarbonate cotransporter 3; Solute carrier family 4 member 7; Solute carrier family 4 sodium bicarbonate cotransporter member 7; anti-SLC4A7 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Supplied in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
~1mg/ml (varies by lot)
Sequence Length
1131
Applicable Applications for anti-SLC4A7 antibody
ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human SLC4A7, corresponding to a region within the internal amino acids.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degrees C. Samples were then incubated with primary Ab(AAA328178 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37 degrees C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue).)
IF (Immunofluorescence)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degrees C. Samples were then incubated with primary Ab(AAA328178 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37 degrees C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue).)
IHC (Immunohiostchemistry)
(AAA328178 at 1/100 staining Rat muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4 degrees C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)
IHC (Immunohiostchemistry)
(AAA328178 at 1/100 staining Rat muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4 degrees C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)
WB (Western Blot)
(Western blot analysis SLC4A7 using HuvEc whole cell lysates)
WB (Western Blot)
(Western blot analysis SLC4A7 using HuvEc whole cell lysates)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
136 kDa
NCBI Official Full Name
sodium bicarbonate cotransporter 3 isoform b
NCBI Official Synonym Full Names
solute carrier family 4 member 7
NCBI Official Symbol
SLC4A7
NCBI Official Synonym Symbols
NBC2; NBC3; SBC2; NBCN1; SLC4A6
NCBI Protein Information
sodium bicarbonate cotransporter 3
UniProt Protein Name
Sodium bicarbonate cotransporter 3
UniProt Gene Name
SLC4A7
UniProt Synonym Gene Names
BT; NBC2; NBC2B; NBC3; NBCn1; SBC2; SLC4A6; Bicarbonate transporter
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Product Notes
The SLC4A7 slc4a7 (Catalog #AAA328178) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SLC4A7 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SLC4A7 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the SLC4A7 slc4a7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLC4A7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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