Rabbit anti-Human SLC8A3 Polyclonal Antibody | anti-SLC8A3 antibody

SLC8A3 Antibody (C-term)

Cat. Num. AAA286089

• Gene Names: SLC8A3; NCX3
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: SLC8A3, Antibody; SLC8A3 Antibody (C-term); Sodium/calcium exchanger 3; Na(+)/Ca(2+)-exchange protein 3; Solute carrier family 8 member 3; SLC8A3; NCX3; anti-SLC8A3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This SLC8A3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 585-614 amino acids from the C-terminal region of human SLC8A3.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 585-614
• Sequence Length: 298

• Applicable Applications for anti-SLC8A3 antibody: IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Function: Mediates the electrogenic exchange of Ca(2+) against Na(+) ions across the cell membrane, and thereby contributes to the regulation of cytoplasmic Ca(2+) levels and Ca(2+)-dependent cellular processes. Contributes to cellular Ca(2+) homeostasis in excitable cells, both in muscle and in brain. In a first phase, voltage-gated channels mediate the rapid increase of cytoplasmic Ca(2+) levels due to release of Ca(2+) stores from the endoplasmic reticulum. SLC8A3 mediates the export of Ca(2+) from the cell during the next phase, so that cytoplasmic Ca(2+) levels rapidly return to baseline. Contributes to Ca(2+) transport during excitation-contraction coupling in muscle. In neurons, contributes to the rapid decrease of cytoplasmic Ca(2+) levels back to baseline after neuronal activation, and thereby contributes to modulate synaptic plasticity, learning and memory (By similarity). Required for normal oligodendrocyte differentiation and for normal myelination (PubMed: ). Mediates Ca(2+) efflux from mitochondria and contributes to mitochondrial Ca(2+) ion homeostasis (By similarity).
• Cellular Location: Cell membrane; Multi-pass membrane protein. Perikaryon {ECO:0000250|UniProtKB:P70549}. Cell projection, dendrite {ECO:0000250|UniProtKB:P70549}. Cell projection, dendritic spine {ECO:0000250|UniProtKB:P70549}. Cell membrane, sarcolemma {ECO:0000250|UniProtKB:S4R2P9}. Cytoplasm, sarcoplasm {ECO:0000250|UniProtKB:S4R2P9}. Cell junction {ECO:0000250|UniProtKB:S4R2P9}. Mitochondrion outer membrane {ECO:0000250|UniProtKB:S4R2P9}; Multi-pass membrane protein ECO:0000250|UniProtKB:S4R2P9}. Cytoplasm, perinuclear region. Endoplasmic reticulum membrane; Multi-pass membrane protein {ECO:0000250|UniProtKB:S4R2P9}. Note=Detected at neuromuscular junctions. {ECO:0000250|UniProtKB:S4R2P9}
• Tissue Location: Isoform 2 is expressed in brain and skeletal muscle. Isoform 3 is expressed in excitable cells of brain, retina and skeletal muscle. Isoform 4 is expressed in skeletal muscle
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohiostchemistry)

(SLC8A3 Antibdy (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SLC8A3 Antibdy (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(SLC8A3 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the SLC8A3 antibody detected the SLC8A3 protein (arrow).)

Related Product Information for anti-SLC8A3 antibody

This gene encodes a member of the sodium/calcium exchanger integral membrane protein family. Three mammalian isoforms in family 8 have been identified. Na+/Ca2+ exchange proteins are involved in maintaining Ca2+ homeostasis in a wide variety of cell types. The protein is regulated by intracellular calcium ions and is found in both the plasma membrane and intracellular organellar membranes, where exchange of Na+ for Ca2+ occurs in an electrogenic manner. Alternative splicing has been observed for this gene and multiple variants have been described.

Product Categories/Family for anti-SLC8A3 antibody

Signal Transduction

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Pulina, M.V., et al. J. Biol. Chem. 281(28):19645-19654(2006)
Gomez-Villafuertes, R., et al. J. Neurosci. 25(47):10822-10830(2005)
Lindgren, R.M., et al. Gene 348, 143-155 (2005) :
Gabellini, N. Mol. Neurobiol. 30(1):91-116(2004)

NCBI and Uniprot Product Information

• NCBI GI #: 194354001
• NCBI GeneID: 6547
• NCBI Accession #: NP_001123889.1; NP_150287.1; NP_489479.1; NP_891977.1; NP_891981.1; NP_892114.1
• NCBI GenBank Nucleotide #: NM_001130417.2
• UniProt Accession #: P57103; Q5K3P6; Q5K3P7; Q8IUE9; Q8IUF0; Q8NFI7; Q96QG1; Q96QG2
• NCBI Official Full Name: sodium/calcium exchanger 3 isoform G
• NCBI Official Synonym Full Names: solute carrier family 8 (sodium/calcium exchanger), member 3
• NCBI Official Symbol: SLC8A3
• NCBI Official Synonym Symbols: NCX3
• NCBI Protein Information: sodium/calcium exchanger 3
• UniProt Protein Name: Sodium/calcium exchanger 3
• UniProt Gene Name: SLC8A3
• UniProt Synonym Gene Names: NCX3
• UniProt Entry Name: NAC3_HUMAN