Rabbit anti-Human SLCO1B3 Polyclonal Antibody | anti-SLCO1B3 antibody

SLCO1B3 Antibody (C-term)

Cat. Num. AAA28785

• Gene Names: SLCO1B3; LST3; HBLRR; LST-2; OATP8; OATP-8; OATP1B3; SLC21A8; LST-3TM13
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: SLCO1B3, Antibody; SLCO1B3 Antibody (C-term); Solute carrier organic anion transporter family member 1B3; Liver-specific organic anion transporter 2; LST-2; Organic anion transporter 8; Organic anion-transporting polypeptide 8; OATP-8; Solute carrier family 21 member 8; SLCO1B3; LST2; OATP1B3; OATP8,SLC21A8; anti-SLCO1B3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This SLCO1B3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 639-668 amino acids from the C-terminal region of human SLCO1B3.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 748

• Applicable Applications for anti-SLCO1B3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:2000
• Antigen Type: Synthetic Peptide
• Antigen Region: 639-668
• Function: Mediates the Na(+)-independent uptake of organic anions such as 17-beta-glucuronosyl estradiol, taurocholate, triiodothyronine (T3), leukotriene C4, dehydroepiandrosterone sulfate (DHEAS), methotrexate and sulfobromophthalein (BSP). Involved in the clearance of bile acids and organic anions from the liver.
• Cellular Location: Basolateral cell membrane; Multi-pass membrane protein
• Tissue Location: Highly expressed in liver, in particular at the basolateral membrane of hepatocytes near the central vein. Not detected in other tissues. Highly expressed in some cancer cell lines derived from colon, pancreas, liver and gall bladder
• Precautions: SLCO1B3 Antibody (C-term) is for research use only
• Function: Mediates the Na(+)-independent uptake of organic anions (PubMed:15159445, PubMed:17412826, PubMed:10779507). Shows broad substrate specificity, can transport both organic anions such as bile acid taurocholate (cholyltaurine) and conjugated steroids (17-betaglucuronosyl estradiol, dehydroepiandrosterone sulfate (DHEAS), and estrone 3-sulfate), as well as eicosanoid leukotriene C4, prostaglandin E2 and L-thyroxine (T4) (PubMed:15159445, PubMed:17412826, PubMed:10779507, PubMed:12568656, PubMed:11159893, PubMed:19129463). Hydrogencarbonate/HCO3(-) acts as the probable counteranion that exchanges for organic anions (PubMed:19129463). Shows a pH-sensitive substrate specificity towards sulfated steroids, taurocholate and T4 which may be ascribed to the protonation state of the binding site and leads to a stimulation of substrate transport in an acidic microenvironment (PubMed:19129463). Involved in the clearance of bile acids and organic anions from the liver (PubMed:22232210). Can take up bilirubin glucuronides from plasma into the liver, contributing to the detoxification-enhancing liver-blood shuttling loop (PubMed:22232210). Transports coproporphyrin I and III, by-products of heme synthesis, and may be involved in their hepatic disposition (PubMed:26383540). May contribute to regulate the transport of organic compounds in testes across the blood-testis-barrier (Probable). Can transport HMG-CoA reductase inhibitors (also known as statins) such as pitavastatin, a clinically important class of hypolipidemic drugs (PubMed:15159445). May play an important role in plasma and tissue distribution of the structurally diverse chemotherapeutic drugs methotrexate and paclitaxel (PubMed:23243220). May also transport antihypertension agents, such as the angiotensin-converting enzyme (ACE) inhibitor prodrug enalapril, and the highly selective angiotensin II AT1-receptor antagonist valsartan, in the liver (PubMed:16627748, PubMed:16624871).
• Cellular Location: Basolateral cell membrane; Multi-pass membrane protein. Basal cell membrane; Multi-pass membrane protein. Note=Localized to the basolateral membrane of hepatocytes (PubMed:10779507). Localized to the basal membrane of Sertoli cells (PubMed:35307651)
• Tissue Location: Highly expressed in liver, in particular at the basolateral membrane of hepatocytes near the central vein (PubMed:10779507, PubMed:15159445). Expressed in the placenta (PubMed:12409283). In testis, primarily localized to the basal membrane of Sertoli cells and weakly expressed in Leydig cells and within the tubules (PubMed:35307651).
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(SLCO1B3 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the SLCO1B3 antibody detected the SLCO1B3 protein (arrow).)

WB (Western Blot)

(All lanes : Anti-SLCO1B3 Antibody (C-term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: SW480 whole cell lysate. Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted bandsize : 77 kDa Blocking/Dilution buffer: 5%NFDM/TBST.)

WB (Western Blot)

(All lanes : Anti-SLCO1B3 Antibody (C-term) at1:2000 dilution Lane 1: NCI-H460 whole cell lysate Lane 2: Hela whole cell lysate. Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted bandsize : 77 kDa Blocking/Dilution buffer: 5%NFDM/TBST.)

WB (Western Blot)

(Anti-SLCO1B3 Antibody (C-term)at 1:1000 dilution + NCI-H460 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 77 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(All lanes : Anti-SLCO1B3 Antibody (C-term) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: PC-3 whole cell lysatesLane 3: SW480 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 77 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(All lanes : Anti-SLCO1B3 Antibody (C-term) at 1:2000 dilutionLane 1: human liver lysatesLane 2: human ovary lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 77 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-SLCO1B3 antibody

SLCO1B3 belongs to the organic anion transporter (OATP)
family. OATPs are involved in the membrane transport of bile acids,
conjugated steroids, thyroid hormone, eicosanoids, peptides, and
numerous drugs in many tissues (Mikkaichi et al., 2004 [PubMed
14993604]).

References

Hu, M., et al. Pharmacogenet. Genomics 20(10):634-637(2010)
Weiner, M., et al. Antimicrob. Agents Chemother. 54(10):4192-4200(2010)
Kang, T.W., et al. Hum. Mol. Genet. 19(18):3672-3678(2010)
Takahashi, N., et al. J. Hum. Genet. (2010) In press :
Justenhoven, C., et al. Breast Cancer Res. Treat. (2010) In press :

NCBI and Uniprot Product Information

• NCBI GI #: 9790233
• NCBI GeneID: 28234
• NCBI Accession #: NP_062818.1
• NCBI GenBank Nucleotide #: NM_019844.3
• UniProt Accession #: Q9NPD5; Q5JAR4; E7EMT8
• NCBI Official Full Name: solute carrier organic anion transporter family member 1B3
• NCBI Official Synonym Full Names: solute carrier organic anion transporter family, member 1B3
• NCBI Official Symbol: SLCO1B3
• NCBI Official Synonym Symbols: LST3; HBLRR; LST-2; OATP8; OATP-8; OATP1B3; SLC21A8; LST-3TM13
• NCBI Protein Information: solute carrier organic anion transporter family member 1B3
• UniProt Protein Name: Solute carrier organic anion transporter family member 1B3
• UniProt Gene Name: SLCO1B3
• UniProt Synonym Gene Names: LST2; OATP1B3; OATP8; SLC21A8; LST-2; OATP-8
• UniProt Entry Name: SO1B3_HUMAN