Rabbit Slingshot-1L Polyclonal Antibody | anti-SSH1 antibody

Slingshot-1L (C-terminal region) Antibody

Cat. Num. AAA71712

• Gene Names: SSH1; SSH1L
• Reactivity: Human, Rat, Mouse
• Applications: Immunohistochemistry, Immunocytochemistry, ELISA, Western Blot
• Synonyms: Slingshot-1L, Antibody; Slingshot-1L (C-terminal region) Antibody; Slingshot-1L (C-terminal region); Slingshot; anti-SSH1 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Specificity: This antibody was affinity purified using SSH1L peptide (without carrier). The antibody detects a 150kDa* protein corresponding to the molecular mass of SSH1L on SDS-PAGE immunoblots of PC12 cells.
• Form/Format: Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-SSH1 antibody: IHC (Immunohistochemistry), ICC (Immunocytochemistry), ELISA, WB (Western Blot)
• Immunogen: A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human SSH1L. This sequence has 100% homology with similar regions of mouse and bovine SSH1L, and has low homology to SSH2L and SSH3L.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

IHC (Immunohiostchemistry)

(Formalin fixed, citric acid treated parafin sections of mouse cerebral cortex. Sections were probed with anti-Slingshot-1L (SP1711) then anti-Rabbit:HRP before detection using DAB. (Image provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).)

WB (Western Blot)

(Western blot of human recombinant SSH1L untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) and rat PC12 cells unstimulated (lanes 5 & 7) or stimulated with calyculin A (lanes 6 & 8). The blots were probed with anti-SSH1L (C-term.) (lanes 1, 2, 5, & 6) or anti-SSH1L (Ser-978) (lanes 3, 4, 7, & 8).)

Related Product Information for anti-SSH1 antibody

Members of the ADF/cofilin (AC) family are actin-severing proteins that regulate actin remodeling during cell motility. Regulation of cofilin activity can occur through serine phosphorylation and dephosphorylation. Activation of cofilin kinases, LIMK1 or LIMK2, leads to phosphorylation of cofilin at serine 3. This phosphorylation disrupts cofilin binding to actin in vitro and in vivo. Multiple phosphatases, Slingshot, PP1, PP2A, PP2B, and chronophin can dephosphorylate Ser-3 and activate actin binding. In mammals, the Slingshot family includes SSH1L, SSH2L, and SSH3L. SSH1L and SSH2L mRNAs are widely expressed, while SSH3L has high expression in epithelial tissues. SSH1L can associate with F-actin and may be the major phosphatase regulating cofilin activity. Disruption of SSH1L expression using RNA interference impairs directional cell migration. Thus, Slingshot phosphatases may be critical for regulating cytoskeletal protein activity and cell motility.

References

Soosairajah, J. et al. (2005) EMBO J. 24(3):473.
Endo, M. et al. (2003) J Neurosci. 23(7):2527.
Niwa, R. et al. (2002) Cell 108:233.

NCBI and Uniprot Product Information

• NCBI GI #: 239047441
• NCBI GeneID: 54434
• NCBI Accession #: NP_001154802.1
• NCBI GenBank Nucleotide #: NM_001161330.1
• UniProt Accession #: Q8WYL5; Q6P6C0; Q8N9A7; Q8WYL3; Q8WYL4; Q9P2P8
• Molecular Weight: 78,978 Da
• NCBI Official Full Name: protein phosphatase Slingshot homolog 1 isoform 2
• NCBI Official Synonym Full Names: slingshot protein phosphatase 1
• NCBI Official Symbol: SSH1
• NCBI Official Synonym Symbols: SSH1L
• NCBI Protein Information: protein phosphatase Slingshot homolog 1
• UniProt Protein Name: Protein phosphatase Slingshot homolog 1
• UniProt Gene Name: SSH1
• UniProt Synonym Gene Names: KIAA1298; SSH1L; SSH-1L; hSSH-1L