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ICC (Immunocytochemistry)
(Anti-Smad2 antibody, AAA45512, ICCICC: HELA Cell)
Rabbit Smad2 Polyclonal Antibody | anti-SMAD2 antibody
Anti-Smad2 antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
Smad2, Antibody; Anti-Smad2 antibody; Mothers against decapentaplegic homolog 2; SMAD family member 2; hMAD 2 antibody; hMAD-2 antibody; hSMAD2 antibody; JV18 1 antibody; JV18 antibody; JV18-1 antibody; JV181 antibody; MAD antibody; MAD homolog 2 antibody; MAD Related Protein 2 antibody; Mad-related protein 2 antibody; MADH2 antibody; MADR2 antibody; MGC22139 antibody; MGC34440 antibody; Mothers Against Decapentaplegic Homolog 2 antibody; mothers against DPP homolog 2 antibody; OTTHUMP00000163489 antibody; Sma and Mad related protein 2 antibody; SMAD 2 antibody; SMAD antibody; SMAD family member 2 antibody; SMAD, mothers against DPP homolog 2 antibody; SMAD2 antibody; SMAD2_HUMAN antibody; anti-SMAD2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
467
Applicable Applications for anti-SMAD2 antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Smad2 (94-112aa DQWDTTGLYSFSEQTRSLD), identical to the related rat and mouse sequences.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ICC (Immunocytochemistry)
(Anti-Smad2 antibody, AAA45512, ICCICC: HELA Cell)
ICC (Immunocytochemistry)
(Anti-Smad2 antibody, AAA45512, ICCICC: HELA Cell)
WB (Western Blot)
(Anti-Smad2 antibody, AAA45512, Western blottingLane 1: Rat Brain Tissue LysateLane 2: HELA Cell LysateLane 3: SMMC Cell LysateLane 4: JURKAT Cell LysateLane 5: U87 Cell LysateLane 6: MCF-7 Cell Lysate)
WB (Western Blot)
(Anti-Smad2 antibody, AAA45512, Western blottingLane 1: Rat Brain Tissue LysateLane 2: HELA Cell LysateLane 3: SMMC Cell LysateLane 4: JURKAT Cell LysateLane 5: U87 Cell LysateLane 6: MCF-7 Cell Lysate)
IHC (Immunohiostchemistry)
(Anti-Smad2 antibody, AAA45512, IHC(P)IHC(P): Human Mammary Cancer Tissue)
IHC (Immunohiostchemistry)
(Anti-Smad2 antibody, AAA45512, IHC(P)IHC(P): Human Mammary Cancer Tissue)
WB (Western Blot)
(Anti-Smad2 antibody, AAA45512, Western blottingRecombinant Protein Detection Source: E.coli derived -recombinant human SHC1, 35.0KD(162aa tag+D424-P578)Lane 1: Recombinant Human SHC1 Proteins 10ngLane 2: Recombinant Human SHC1 Proteins 5ngLane 3: Recombinant Human SHC1 Proteins 2.5ng)
WB (Western Blot)
(Anti-Smad2 antibody, AAA45512, Western blottingRecombinant Protein Detection Source: E.coli derived -recombinant human SHC1, 35.0KD(162aa tag+D424-P578)Lane 1: Recombinant Human SHC1 Proteins 10ngLane 2: Recombinant Human SHC1 Proteins 5ngLane 3: Recombinant Human SHC1 Proteins 2.5ng)
Related Product Information for anti-SMAD2 antibody
Description: Rabbit IgG polyclonal antibody for Mothers against decapentaplegic homolog 2(SMAD2) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.
Background: Smad2(Mothers against decapentaplegic homolog 2), also known as MADR2, MADH2, SMAD family member 2 or SMAD2, is a protein that in humans is encoded by the SMAD2 gene. MAD homolog 2 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic'(Mad) and the C. elegans gene Sma. Eppert et al.(1996) mapped the MADR2 gene close to DPC4 at 18q21, a region which is frequently deleted in colorectal cancers. Riggins et al.(1996) mapped the human MADH2 gene to 18q21. Nakao et al.(1997) refined the localization of the SMAD2 gene to 18q21.1, approximately 3 Mb proximal to DPC4, by fluorescence in situ hybridization. SMAD2 mediates the signal of the transforming growth factor(TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation(SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors.
Background: Smad2(Mothers against decapentaplegic homolog 2), also known as MADR2, MADH2, SMAD family member 2 or SMAD2, is a protein that in humans is encoded by the SMAD2 gene. MAD homolog 2 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic'(Mad) and the C. elegans gene Sma. Eppert et al.(1996) mapped the MADR2 gene close to DPC4 at 18q21, a region which is frequently deleted in colorectal cancers. Riggins et al.(1996) mapped the human MADH2 gene to 18q21. Nakao et al.(1997) refined the localization of the SMAD2 gene to 18q21.1, approximately 3 Mb proximal to DPC4, by fluorescence in situ hybridization. SMAD2 mediates the signal of the transforming growth factor(TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation(SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
48,956 Da
NCBI Official Full Name
Mothers against decapentaplegic homolog 2
NCBI Official Synonym Full Names
SMAD family member 2
NCBI Official Symbol
SMAD2
NCBI Official Synonym Symbols
JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
NCBI Protein Information
mothers against decapentaplegic homolog 2; MAD homolog 2; mother against DPP homolog 2; Sma- and Mad-related protein 2; SMAD, mothers against DPP homolog 2
UniProt Protein Name
Mothers against decapentaplegic homolog 2
UniProt Gene Name
SMAD2
UniProt Synonym Gene Names
MADH2; MADR2; MAD homolog 2; Mothers against DPP homolog 2; hMAD-2; SMAD 2; Smad2; hSMAD2
UniProt Entry Name
SMAD2_HUMAN
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Product Notes
The SMAD2 smad2 (Catalog #AAA45512) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Smad2 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Smad2 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the SMAD2 smad2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Smad2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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