IP (Immunoprecipitation)
(SMAD3 was immunoprecipitated using:Lane A:0.5 mg A549 Whole Cell Lysate0.5 uL anti-SMAD3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-SMAD3 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 48 kDaObserved band size: 57 kDa)
Rabbit Smad3 Polyclonal Antibody | anti-Smad3 antibody
Anti-Smad3 Antibody, Rabbit Polyclonal
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
HumanPredicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
Smad3, Antibody; Anti-Smad3 Antibody, Rabbit Polyclonal; SMAD3 Antibody, Rabbit PAb, Antigen Affinity Purified; SMAD family member 3; Anti-HSPC193 Antibody; Anti-HsT17436 Antibody; Anti-JV15-2 Antibody; Anti-LDS1C Antibody; Anti-LDS3 Antibody; Anti-MADH3 Antibody; anti-Smad3 antibody
Host
Rabbit
Reactivity
Human
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human Smad3
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-Smad3 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to the center region of the human SMAD3
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with A synthetic peptide corresponding to the center region of the human SMAD3, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(SMAD3 was immunoprecipitated using:Lane A:0.5 mg A549 Whole Cell Lysate0.5 uL anti-SMAD3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-SMAD3 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 48 kDaObserved band size: 57 kDa)
IP (Immunoprecipitation)
(SMAD3 was immunoprecipitated using:Lane A:0.5 mg A549 Whole Cell Lysate0.5 uL anti-SMAD3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-SMAD3 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 48 kDaObserved band size: 57 kDa)
WB (Western Blot)
(Anti-SMAD3 rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:48 kDaObserved band size:57 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-SMAD3 rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:48 kDaObserved band size:57 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-Smad3 antibody
SMAD3 belongs to the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD3 is involved in cell signalling. It modulates signals of activin and TGFbeta's. Binding of SMAD3 with SMAD4 enables its transmigration into the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD3 is a receptor-regulated SMAD (R-SMAD). In mice, mutation of SMAD3 has been linked to colorectal adenocarcinoma, increased systemic inflammation, and accelerated wound healing. Increased SMAD3 activity has been implicated in the pathogenesis of scleroderma. Smad3 is also a multifaceted regulator in adipose physiology and the pathogenesis of obesity and type 2 diabetes.
Product Categories/Family for anti-Smad3 antibody
References
Tan. et al., 2011, Diabetes. 60 (2): 464-76. Yang X. et al., 1999, Nat Cell Biol. 1 (5): 260-6. Zhu Y. et al., 1998, Cell. 94 (6): 703-14. Feng X. et al., 1996, Nature. 383 (6596): 168-72.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
48,081 Da
NCBI Official Full Name
mothers against decapentaplegic homolog 3 isoform 2
NCBI Official Synonym Full Names
SMAD family member 3
NCBI Official Symbol
SMAD3
NCBI Official Synonym Symbols
LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
NCBI Protein Information
mothers against decapentaplegic homolog 3; mad3; hMAD-3; hSMAD3; MAD homolog 3; mad homolog JV15-2; mad protein homolog; mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD, mothers against DPP homolog 3; MAD, mothers against decapentapleg
UniProt Protein Name
Mothers against decapentaplegic homolog 3
UniProt Gene Name
SMAD3
UniProt Synonym Gene Names
MADH3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; SMAD 3; Smad3; hSMAD3
UniProt Entry Name
SMAD3_HUMAN
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Product Notes
The Smad3 smad3 (Catalog #AAA254226) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Smad3 Antibody, Rabbit Polyclonal reacts with Human Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's Smad3 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the Smad3 smad3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Smad3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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