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WB (Western Blot)
(Detection of mouse SMAR1 by western blot. Samples: Whole cell lysate (25 ug) from NIH 3T3, TCMK-1, CT26, CH27, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMAR1 antibody (AAA210857 lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse SMAR1/BANP Polyclonal Antibody | anti-SMAR1/BANP antibody
Rabbit anti-SMAR1/BANP Antibody, Affinity Purified
Gene Names
BANP; BEND1; SMAR1; SMARBP1
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
SMAR1/BANP, Antibody; Rabbit anti-SMAR1/BANP Antibody, Affinity Purified; BEND1; SMARBP1; SMAR1; scaffold/matrix-associated region-1-binding protein; Btg3-associated nuclear protein; BEN domain-containing protein 1; protein BANP; Protein BANP; BTG3 associated nuclear protein; anti-SMAR1/BANP antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-SMAR1/BANP antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Bovine
Immunogen
Between 469 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse SMAR1 by western blot. Samples: Whole cell lysate (25 ug) from NIH 3T3, TCMK-1, CT26, CH27, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMAR1 antibody (AAA210857 lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of mouse SMAR1 by western blot. Samples: Whole cell lysate (25 ug) from NIH 3T3, TCMK-1, CT26, CH27, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMAR1 antibody (AAA210857 lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human SMAR1 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, Jurkat, MDA-MB-231, RKO, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMAR1 antibody (AAA210857 lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human SMAR1 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, Jurkat, MDA-MB-231, RKO, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMAR1 antibody (AAA210857 lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human SMAR1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMAR1 antibody (AAA210857 lot 4) used for IP at 6 ug per reaction. SMAR1 was also immunoprecipitated by a previous lot of this antibody (AAA210857 lot 3). For blotting immunoprecipitated SMAR1, AAA210857 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human SMAR1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMAR1 antibody (AAA210857 lot 4) used for IP at 6 ug per reaction. SMAR1 was also immunoprecipitated by a previous lot of this antibody (AAA210857 lot 3). For blotting immunoprecipitated SMAR1, AAA210857 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemistry)
(Detection of human SMAR1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibodies: Affinity purified rabbit anti-SMAR1 (AAA210857 Lot 4) used at dilution of 1:5,000 (0.2 ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human SMAR1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibodies: Affinity purified rabbit anti-SMAR1 (AAA210857 Lot 4) used at dilution of 1:5,000 (0.2 ug/ml). Detection: DAB)
Related Product Information for anti-SMAR1/BANP antibody
SMAR1/BANP was identified as a protein that binds the scaffold/matrix associated region of the T-cell receptor beta locus (SMAR1) and as a protein that interacts with BTG3, a member of structurally related proteins that regulate cell proliferation (BANP- BTG-associated nuclear protein). SMAR1/BANP is a DNA binding protein that is reported to be a tumor suppressor and transcriptional repressor. SMAR1/BANP can down-regulate cyclin D1 transcription by binding to the cyclin D1 promoter and through its interaction with HDAC1 (histone deacetylase 1). SMAR1 has also been shown to interact with p53 and modulate its activity.
Product Categories/Family for anti-SMAR1/BANP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55,502 Da
NCBI Official Full Name
protein BANP isoform a
NCBI Official Synonym Full Names
BTG3 associated nuclear protein
NCBI Official Symbol
BANP
NCBI Official Synonym Symbols
BEND1; SMAR1; SMARBP1
NCBI Protein Information
protein BANP; BEN domain-containing protein 1; scaffold/matrix-associated region-1-binding protein
UniProt Protein Name
Protein BANP
UniProt Gene Name
BANP
UniProt Synonym Gene Names
BEND1; SMAR1
UniProt Entry Name
BANP_HUMAN
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Product Notes
The SMAR1/BANP banp (Catalog #AAA210857) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-SMAR1/BANP Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SMAR1/BANP can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SMAR1/BANP banp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMAR1/BANP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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