WB (Western Blot)
(Detection of human SMARCA3 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SMARCA3 antibody AAA210827 (lot AAA210827-2) used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. SMARCA3 was also immunoprecipitated by a previous lot (lot AAA210827-1) of this antibody. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).)
Rabbit anti-Human, Mouse SMARCA3 Polyclonal Antibody | anti-SMARCA3 antibody
Rabbit anti-SMARCA3 Antibody, Affinity Purified
Gene Names
HLTF; ZBU1; HLTF1; RNF80; HIP116; SNF2L3; HIP116A; SMARCA3
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
SMARCA3, Antibody; Rabbit anti-SMARCA3 Antibody, Affinity Purified; RNF80; SMARCA3; ZBU1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 3; sucrose nonfermenting-like 3; sucrose nonfermenting protein 2-like 3; SNF2-like 3; RING-type E3 ubiquitin transferase HLTF; RING finger protein 80; HLTF1; HIP116A; HIP116; helicase-like transcription factor; DNA-binding protein/plasminogen activator inhibitor-1 regulator; DNA-binding protein/plasminogen activator inhibitor 1 regulator; SNF2L3; Helicase-like transcription factor; helicase like transcription factor; anti-SMARCA3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-SMARCA3 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 625 and 675
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human SMARCA3 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SMARCA3 antibody AAA210827 (lot AAA210827-2) used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. SMARCA3 was also immunoprecipitated by a previous lot (lot AAA210827-1) of this antibody. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).)
WB (Western Blot)
(Detection of human SMARCA3 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SMARCA3 antibody AAA210827 (lot AAA210827-2) used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. SMARCA3 was also immunoprecipitated by a previous lot (lot AAA210827-1) of this antibody. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).)
WB (Western Blot)
(Detection of mouse SMARCA3 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-SMARCA3 antibody AAA210827 (lot AAA210827-2) used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of mouse SMARCA3 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-SMARCA3 antibody AAA210827 (lot AAA210827-2) used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-SMARCA3 antibody
The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SMARCA3 also known as HLTF (helicase-like transcription factor) has been shown to bind DNA promoter elements and stimulate transcription in cooperation with Sp1and Sp3 transcription factors.
Product Categories/Family for anti-SMARCA3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
99,919 Da
NCBI Official Full Name
helicase-like transcription factor
NCBI Official Synonym Full Names
helicase-like transcription factor
NCBI Official Symbol
HLTF
NCBI Official Synonym Symbols
ZBU1; HLTF1; RNF80; HIP116; SNF2L3; HIP116A; SMARCA3
NCBI Protein Information
helicase-like transcription factor; DNA-binding protein/plasminogen activator inhibitor 1 regulator; DNA-binding protein/plasminogen activator inhibitor-1 regulator; RING finger protein 80; SNF2-like 3; SWI/SNF related, matrix associated, actin dependent
UniProt Protein Name
Helicase-like transcription factor
UniProt Gene Name
HLTF
UniProt Synonym Gene Names
HIP116A; RNF80; SMARCA3; SNF2L3; ZBU1
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Product Notes
The SMARCA3 hltf (Catalog #AAA210827) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-SMARCA3 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SMARCA3 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SMARCA3 hltf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMARCA3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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