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WB (Western Blot)
(Detection of human SMARCB1/SNF5 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, Jurkat, K-562, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMARCB1/SNF5 antibody (AAA211311 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Rabbit anti-Human, Mouse SMARCB1/SNF5 Polyclonal Antibody | anti-SMARCB1/SNF5 antibody
Rabbit anti-SMARCB1/SNF5 Antibody, Affinity Purified
Gene Names
SMARCB1; RDT; INI1; SNF5; Snr1; BAF47; MRD15; RTPS1; Sfh1p; hSNFS; SNF5L1; SWNTS1; PPP1R144
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
SMARCB1/SNF5, Antibody; Rabbit anti-SMARCB1/SNF5 Antibody, Affinity Purified; SNF5L1; Sfh1p; SNF5; SNF5 homolog; Snr1; sucrose nonfermenting, yeast, homolog-like 1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1; RTPS1; SWNTS1; integrase interactor 1 protein; SWI/SNF-related matrix-associated protein; CSS3; MRD15; BRG1-associated factor 47; RDT; hSNF5; hSNFS; INI1; malignant rhabdoid tumor suppressor; PPP1R144; protein phosphatase 1, regulatory subunit 144; BAF47; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1; anti-SMARCB1/SNF5 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-SMARCB1/SNF5 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
X. laevis, X. tropicalis, Chicken, Bovine
Immunogen
Between 100 and 150
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human SMARCB1/SNF5 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, Jurkat, K-562, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMARCB1/SNF5 antibody (AAA211311 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of human SMARCB1/SNF5 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, Jurkat, K-562, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMARCB1/SNF5 antibody (AAA211311 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of mouse SMARCB1/SNF5 by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, and CH27 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMARCB1/SNF5 antibody (AAA211311 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of mouse SMARCB1/SNF5 by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, and CH27 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMARCB1/SNF5 antibody (AAA211311 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human SMARCB1/SNF5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMARCB1/SNF5 antibody (AAA211311 lot 3) used for IP at 6 ug per reaction. SMARCB1/SNF5 was also immunoprecipitated by a previous lot of this antibody (AAA211311 lot 2) and a second antibody against a different epitope of SMARCB1/SNF5 (BL708). For blotting immunoprecipitated SMARCB1/SNF5, AAA211311 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human SMARCB1/SNF5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMARCB1/SNF5 antibody (AAA211311 lot 3) used for IP at 6 ug per reaction. SMARCB1/SNF5 was also immunoprecipitated by a previous lot of this antibody (AAA211311 lot 2) and a second antibody against a different epitope of SMARCB1/SNF5 (BL708). For blotting immunoprecipitated SMARCB1/SNF5, AAA211311 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human SMARCB1/SNF5 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti SMARCB1/SNF5 (AAA211311 Lot 3) used at a dilution of 1:2,000 (0.5ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human SMARCB1/SNF5 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti SMARCB1/SNF5 (AAA211311 Lot 3) used at a dilution of 1:2,000 (0.5ug/ml). Detection: DAB)
Related Product Information for anti-SMARCB1/SNF5 antibody
The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SMARCA3 also known as HLTF (helicase-like transcription factor) has been shown to bind DNA promoter elements and stimulate transcription in cooperation with Sp1and Sp3 transcription factors.
Product Categories/Family for anti-SMARCB1/SNF5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,158 Da
NCBI Official Full Name
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1 isoform a
NCBI Official Synonym Full Names
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1
NCBI Official Symbol
SMARCB1
NCBI Official Synonym Symbols
RDT; INI1; SNF5; Snr1; BAF47; MRD15; RTPS1; Sfh1p; hSNFS; SNF5L1; SWNTS1; PPP1R144
NCBI Protein Information
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1; hSNF5; SNF5 homolog; BRG1-associated factor 47; integrase interactor 1 protein; malignant rhabdoid tumor suppressor; SWI/SNF-related matrix-associated protein;
UniProt Protein Name
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1
UniProt Gene Name
SMARCB1
UniProt Synonym Gene Names
BAF47; INI1; SNF5L1; BAF47; hSNF5
UniProt Entry Name
SNF5_HUMAN
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Product Notes
The SMARCB1/SNF5 smarcb1 (Catalog #AAA211311) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-SMARCB1/SNF5 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SMARCB1/SNF5 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SMARCB1/SNF5 smarcb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMARCB1/SNF5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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