FCM/FACS (Flow Cytometry)
(Dilution: FC: 1:10~50SMARCC1 Antibody (N-term) (Cat. #AAA291030) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit SMARCC1 Polyclonal Antibody | anti-SMARCC1 antibody
SMARCC1 Antibody (N-term)
Gene Names
SMARCC1; Rsc8; SRG3; SWI3; BAF155; CRACC1
Reactivity
HumanPredicted Reactivity: Mouse
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SMARCC1, Antibody; SMARCC1 Antibody (N-term); SWI/SNF complex subunit SMARCC1; BRG1-associated factor 155; BAF155; SWI/SNF complex 155 kDa subunit; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1; SMARCC1; anti-SMARCC1 antibody
Host
Rabbit
Reactivity
Human
Predicted Reactivity: Mouse
Predicted Reactivity: Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
This SMARCC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 268-297 amino acids from the N-terminal region of human SMARCC1.
Purity/Purification
Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applicable Applications for anti-SMARCC1 antibody
WB (Western Blot), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry)
Antigen Region
268-297
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Dilution: FC: 1:10~50SMARCC1 Antibody (N-term) (Cat. #AAA291030) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Dilution: FC: 1:10~50SMARCC1 Antibody (N-term) (Cat. #AAA291030) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Dilution: IHC-P 1:50~100SMARCC1 Antibody (N-term) (Cat. #AAA291030) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SMARCC1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Dilution: IHC-P 1:50~100SMARCC1 Antibody (N-term) (Cat. #AAA291030) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SMARCC1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Dilution: WB 1:1000Western blot analysis of SMARCC1 Antibody (N-term) (Cat. #AAA291030) in K562 cell line lysates (35ug/lane).SMARCC1 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Dilution: WB 1:1000Western blot analysis of SMARCC1 Antibody (N-term) (Cat. #AAA291030) in K562 cell line lysates (35ug/lane).SMARCC1 (arrow) was detected using the purified Pab.)
Related Product Information for anti-SMARCC1 antibody
SMARCC1 is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors.
Product Categories/Family for anti-SMARCC1 antibody
References
So, H.C., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (1), 103-113 (2010) Andersen, C.L., et al. Br. J. Cancer 100 (3):511-523 (2009)Heeboll, S., et al. Histol. Histopathol. 23 (9):1069-1076 (2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted MW: 123kDa
NCBI Official Full Name
SWI/SNF complex subunit SMARCC1
NCBI Official Synonym Full Names
SWI/SNF related, matrix associated, actin dependent regulator of chromatin subfamily c member 1
NCBI Official Symbol
SMARCC1
NCBI Official Synonym Symbols
Rsc8; SRG3; SWI3; BAF155; CRACC1
NCBI Protein Information
SWI/SNF complex subunit SMARCC1
UniProt Protein Name
SWI/SNF complex subunit SMARCC1
UniProt Gene Name
SMARCC1
UniProt Synonym Gene Names
BAF155; BAF155
UniProt Entry Name
SMRC1_HUMAN
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Product Notes
The SMARCC1 smarcc1 (Catalog #AAA291030) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SMARCC1 Antibody (N-term) reacts with Human Predicted Reactivity: Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SMARCC1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the SMARCC1 smarcc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMARCC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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