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IHC (Immunohistochemistry)
(AAA328932 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Rabbit SMC2 Polyclonal Antibody | anti-SMC2 antibody
SMC2 Antibody
Gene Names
SMC2; CAPE; CAP-E; SMC-2; SMC2L1
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig(88%), Zebrafish(100%), Bovine(88%), Horse(100%), Sheep(88%), Rabbit(88%), Dog(100%), Xenopus(100%)
Applications
ELISA, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
SMC2, Antibody; SMC2 Antibody; CAP E; CAPE; Chromosome associated protein E; Chromosome-associated protein E; FLJ10093; hCAP E; hCAP-E; hCAPE; PRO0324; SMC 2; SMC protein 2; SMC-2; SMC2; SMC2 L1; SMC2_HUMAN; SMC2L1; Structural maintenance of chromosomes (SMC) family member chromosome associated protein E; Structural maintenance of chromosomes 2; Structural maintenance of chromosomes 2 like 1; Structural maintenance of chromosomes 2 yeast like 1; Structural maintenance of chromosomes protein 2; XCAP E homolog; XCAP-E homolog; XCAPE homolog; anti-SMC2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(88%), Zebrafish(100%), Bovine(88%), Horse(100%), Sheep(88%), Rabbit(88%), Dog(100%), Xenopus(100%)
Predicted Reactivity: Pig(88%), Zebrafish(100%), Bovine(88%), Horse(100%), Sheep(88%), Rabbit(88%), Dog(100%), Xenopus(100%)
Clonality
Polyclonal
Isotype
IgG
Specificity
SMC2 Antibody detects endogenous levels of total SMC2.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
1197
Applicable Applications for anti-SMC2 antibody
ELISA, WB (Western Blot)
Immunogen
A synthesized peptide derived from human SMC2, corresponding to a region within the internal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohistochemistry)
(AAA328932 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemistry)
(AAA328932 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(AAA328932 at 1/100 staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(AAA328932 at 1/100 staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(AAA328932 at 1/100 staining Rat colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(AAA328932 at 1/100 staining Rat colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from Hela cells, using SMC2 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Hela cells, using SMC2 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-SMC2 antibody
Function: Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
Subcellular Location: Nucleus. Cytoplasm. Chromosome. Note: In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.
Subunit Structure: Forms a heterodimer with SMC4. Component of the condensin complex, which contains the SMC2 and SMC4 heterodimer, and three non SMC subunits that probably regulate the complex: BRRN1/CAPH, CNAP1/CAPD2 and CAPG. Interacts with BRD4 (isoform B), leading to insulate chromatin from DNA damage response pathway.
Similarity: The SMC hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC4, forming a V-shaped heterodimer.Belongs to the SMC family. SMC2 subfamily.
Subcellular Location: Nucleus. Cytoplasm. Chromosome. Note: In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.
Subunit Structure: Forms a heterodimer with SMC4. Component of the condensin complex, which contains the SMC2 and SMC4 heterodimer, and three non SMC subunits that probably regulate the complex: BRRN1/CAPH, CNAP1/CAPD2 and CAPG. Interacts with BRD4 (isoform B), leading to insulate chromatin from DNA damage response pathway.
Similarity: The SMC hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC4, forming a V-shaped heterodimer.Belongs to the SMC family. SMC2 subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)135kD.
Predicted Molecular Weight: (Calculated)136kDa.
Predicted Molecular Weight: (Calculated)136kDa.
NCBI Official Full Name
structural maintenance of chromosomes protein 2
NCBI Official Synonym Full Names
structural maintenance of chromosomes 2
NCBI Official Symbol
SMC2
NCBI Official Synonym Symbols
CAPE; CAP-E; SMC-2; SMC2L1
NCBI Protein Information
structural maintenance of chromosomes protein 2
UniProt Protein Name
Structural maintenance of chromosomes protein 2
UniProt Gene Name
SMC2
UniProt Synonym Gene Names
CAPE; SMC2L1
UniProt Entry Name
SMC2_HUMAN
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Product Notes
The SMC2 smc2 (Catalog #AAA328932) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SMC2 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig(88%), Zebrafish(100%), Bovine(88%), Horse(100%), Sheep(88%), Rabbit(88%), Dog(100%), Xenopus(100%) and may cross-react with other species as described in the data sheet. AAA Biotech's SMC2 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the SMC2 smc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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