Rabbit anti-Human SMN1 Polyclonal Antibody | anti-SMN1 antibody

SMN1 Antibody

Cat. Num. AAA232851

• Gene Names: SMN1; SMA; SMN; SMA1; SMA2; SMA3; SMA4; SMA@; SMNT; BCD541; GEMIN1; TDRD16A; T-BCD541
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
• Purity: >95%, Protein G purified
• Synonyms: SMN1, Antibody; SMN1 Antibody; Survival motor neuron protein; Component of gems 1; Gemin-1; SMN1; SMN; SMNT; SMN2; SMNC; anti-SMN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 294

• Applicable Applications for anti-SMN1 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Immunogen: Recombinant Human Survival motor neuron protein protein (3-194AA)
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Conjugation: Non-conjugated
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-137200 / sc-15320 / sc-7804
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

IHC (Immunohistochemisry)

(Immunofluorescent analysis of HepG2 cells using AAA232851 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

IHC (Immunohiostchemistry)

(Immunohistochemistry of paraffin-embedded human testis tissue using AAA232851 at dilution of 1:100)

WB (Western Blot)

(Western BlotPositive WB detected in: A549 whole cell lysate,Hela whole cell lysateAll lanes: SMN1 antibody at 2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 32,29,31,28 KDaObserved band size: 40,60 KDa)

Related Product Information for anti-SMN1 antibody

The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. Ensures the correct splicing of U12 intron-containing genes that may be important for normal motor and proprioceptive neurons development. Also required for resolving RNA-DNA hybrids created by RNA polymerase II, that form R-loop in transcription terminal regions, an important step in proper transcription termination. May also play a role in the metabolism of small nucleolar ribonucleoprotein (snoRNPs).

NCBI and Uniprot Product Information

• NCBI GI #: 4507091
• NCBI GeneID: 6606
• NCBI Accession #: NP_000335.1
• NCBI GenBank Nucleotide #: NM_000344.3
• UniProt Accession #: Q16637; Q13119; Q549U5; Q96J51; A8K0V4
• Molecular Weight: 27,135 Da
• NCBI Official Full Name: survival motor neuron protein isoform d
• NCBI Official Synonym Full Names: survival of motor neuron 1, telomeric
• NCBI Official Symbol: SMN1
• NCBI Official Synonym Symbols: SMA; SMN; SMA1; SMA2; SMA3; SMA4; SMA@; SMNT; BCD541; GEMIN1; TDRD16A; T-BCD541
• NCBI Protein Information: survival motor neuron protein
• UniProt Protein Name: Survival motor neuron protein
• UniProt Gene Name: SMN1
• UniProt Synonym Gene Names: SMN; SMNT
• UniProt Entry Name: SMN_HUMAN