FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HepG2 cells using anti-SMOC1 antibody (AAA127472).Overlay histogram showing HepG2 cells stained with AAA127472 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SMOC1 Antibody (AAA127472, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
Rabbit anti-Human SMOC1 Polyclonal Antibody | anti-SMOC1 antibody
Anti-SMOC1 Antibody Picoband
Gene Names
SMOC1; OAS
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Functional Assay, ELISA
Purity
Immunogen affinity purified.
Synonyms
SMOC1, Antibody; Anti-SMOC1 Antibody Picoband; Chromaffin granule amine transporter; SRY-box 15; Protein SOX-15; Protein SOX-12; Protein SOX-20; SOX15; SOX12; SOX20; SOX26; SOX27; anti-SMOC1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Widely expressed in fetal and adult tissues examined, highest level found in fetal spinal cord and adult brain and testis.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Applicable Applications for anti-SMOC1 antibody
WB (Western Blot), FCM/FACS (Flow Cytometry), ELISA
Immunogen
E coli-derived human SMOC1 recombinant protein (Position: H27-V434).
Subcellular Localization
Nucleus
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
Binds to the 5'-AACAAT-3' sequence.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HepG2 cells using anti-SMOC1 antibody (AAA127472).Overlay histogram showing HepG2 cells stained with AAA127472 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SMOC1 Antibody (AAA127472, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HepG2 cells using anti-SMOC1 antibody (AAA127472).Overlay histogram showing HepG2 cells stained with AAA127472 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SMOC1 Antibody (AAA127472, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
WB (Western Blot)
(Figure 1. Western blot analysis of SMOC1 using anti-SMOC1 antibody (AAA127472).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human 293T whole cell lysates,Lane 2: human Hela whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMOC1 antigen affinity purified polyclonal antibody (#AAA127472) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SMOC1 at approximately 70 kDa. The expected band size for SMOC1 is at 48 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of SMOC1 using anti-SMOC1 antibody (AAA127472).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human 293T whole cell lysates,Lane 2: human Hela whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMOC1 antigen affinity purified polyclonal antibody (#AAA127472) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SMOC1 at approximately 70 kDa. The expected band size for SMOC1 is at 48 kDa.)
Related Product Information for anti-SMOC1 antibody
The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Product Categories/Family for anti-SMOC1 antibody
References
1. Abouzeid, H., Boisset, G., Favez, T., Youssef, M., Marzouk, I., Shakankiry, N., Bayoumi, N., Descombes, P., Agosti, C., Munier, F. L., Schorderet, D. F. Mutations in the SPARC-related modular calcium-binding protein 1 gene, SMOC1, cause Waardenburg anophthalmia syndrome. Am. J. Hum. Genet. 88: 92-98, 2011. 2. Hamanoue, H., Megarbane, A., Tohma, T., Nishimura, A., Mizuguchi, T., Saitsu, H., Sakai, H., Miura, S., Toda, T., Miyake, N., Niikawa, N., Yoshiura, K., Hirahara, F., Matsumoto, N. A locus for ophthalmo-acromelic syndrome mapped to 10p11.23. Am. J. Med. Genet. 149A: 336-342, 2009.3. Okada, I., Hamanoue, H., Terada, K., Tohma, T., Megarbane, A., Chouery, E., Abou-Ghoch, J., Jalkh, N., Cogulu, O., Ozkinay, F., Horie, K., Takeda, J., and 16 others. SMOC1 is essential for ocular and limb development in humans and mice. Am. J. Hum. Genet. 88: 30-41, 2011.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,163 Da
NCBI Official Full Name
SPARC-related modular calcium-binding protein 1 isoform 1
NCBI Official Synonym Full Names
SPARC related modular calcium binding 1
NCBI Official Symbol
SMOC1
NCBI Official Synonym Symbols
OAS
NCBI Protein Information
SPARC-related modular calcium-binding protein 1; secreted modular calcium-binding protein 1
UniProt Protein Name
SPARC-related modular calcium-binding protein 1
UniProt Gene Name
SMOC1
UniProt Synonym Gene Names
SMOC-1
UniProt Entry Name
SMOC1_HUMAN
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Product Notes
The SMOC1 smoc1 (Catalog #AAA127472) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SMOC1 Antibody Picoband reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SMOC1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the SMOC1 smoc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMOC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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