IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human Testis tissue reacted with hSMURF1-W81 , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit SMURF1 Polyclonal Antibody | anti-SMURF1 antibody
SMURF1 Antibody (N-term)
Reactivity
Human, mouse (Predicted Reactivity: Xenopus, Zebrafish)
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SMURF1, Antibody; SMURF1 Antibody (N-term); E3 ubiquitin-protein ligase SMURF1; hSMURF1; 632-; SMAD ubiquitination regulatory factor 1; SMAD-specific E3 ubiquitin-protein ligase 1; SMURF1; KIAA1625; anti-SMURF1 antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Xenopus, Zebrafish)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This SMURF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-96 amino acids from the N-terminal region of human SMURF1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
66-96
Sequence Length
731
Applicable Applications for anti-SMURF1 antibody
WB (Western Blot), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human Testis tissue reacted with hSMURF1-W81 , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human Testis tissue reacted with hSMURF1-W81 , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with hSMURF1-W81 , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with hSMURF1-W81 , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of anti-SMURF1 Pab in mouse kidney tissue lysate (35ug/lane). SMURF1(arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of anti-SMURF1 Pab in mouse kidney tissue lysate (35ug/lane). SMURF1(arrow) was detected using the purified Pab.)
Related Product Information for anti-SMURF1 antibody
Members of the transforming growth factor-beta (TGFB) family signal through type I and type II serine/threonine-kinase receptors, which in turn activate the SMAD signaling pathway. Bone morphogenetic protein (BMP) receptors target SMAD1, SMAD5, and SMAD8, whereas receptors for activin and TGFB (e.g., ACVR1 and TGFBR1, respectively) target SMAD2 and SMAD3. Phosphorylation of these receptor-regulated SMADs induces their association with the common-partner SMAD, SMAD4. Smurf1, a HECT domain E3 ubiquitin ligase, regulates cell polarity and protrusive activity and is required to maintain the transformed morphology and motility of a tumor cell. Atypical protein kinase C-zeta (PKC2), an effector of the Cdc42/Rac1-PAR6 polarity complex, recruits Smurf1 to cellular protrusions, where it controlled the local level of RhoA. Smurf1 thus links the polarity complex to degradation of RhoA in lamellipodia and filopodia to prevent RhoA signaling during dynamic membrane movements.
Product Categories/Family for anti-SMURF1 antibody
References
Tajima, Y., et al., J. Biol. Chem. 278(12):10716-10721 (2003). Suzuki, C., et al., J. Biol. Chem. 277(42):39919-39925 (2002). Ebisawa, T., et al., J. Biol. Chem. 276(16):12477-12480 (2001). Zhu, H., et al., Nature 400(6745):687-693 (1999). Lambris, J., et al., J. Immunol. Methods 27(1):55-59 (1979).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
86114
NCBI Official Full Name
E3 ubiquitin-protein ligase SMURF1 isoform 3
NCBI Official Synonym Full Names
SMAD specific E3 ubiquitin protein ligase 1
NCBI Official Symbol
SMURF1
NCBI Protein Information
E3 ubiquitin-protein ligase SMURF1
UniProt Protein Name
E3 ubiquitin-protein ligase SMURF1
UniProt Gene Name
SMURF1
UniProt Synonym Gene Names
KIAA1625; hSMURF1
UniProt Entry Name
SMUF1_HUMAN
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Product Notes
The SMURF1 smurf1 (Catalog #AAA284499) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 66-96. The SMURF1 Antibody (N-term) reacts with Human, mouse (Predicted Reactivity: Xenopus, Zebrafish) and may cross-react with other species as described in the data sheet. AAA Biotech's SMURF1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the SMURF1 smurf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMURF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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