FCM/FACS (Flow Cytometry)
(SNRPD1 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit SNRPD1 Polyclonal Antibody | anti-SNRPD1 antibody
SNRPD1 Antibody (Center)
Gene Names
SNRPD1; SMD1; SNRPD; Sm-D1; HsT2456
Reactivity
HumanPredicted Reactivity: Bovine, Monkey, Mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SNRPD1, Antibody; SNRPD1 Antibody (Center); Small nuclear ribonucleoprotein Sm D1; Sm-D1; Sm-D autoantigen; snRNP core protein D1; SNRPD1; anti-SNRPD1 antibody
Host
Rabbit
Reactivity
Human
Predicted Reactivity: Bovine, Monkey, Mouse
Predicted Reactivity: Bovine, Monkey, Mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This SNRPD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-78 amino acids from the Central region of human SNRPD1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Positions
46-78
Sequence Length
119
Applicable Applications for anti-SNRPD1 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Cellular Location
Cytoplasm, cytosol. Nucleus. Note=SMN-mediated assembly into core snRNPs occurs in the cytosol before SMN- mediated transport to the nucleus to be included in spliceosomes
Function
Plays role in pre-mRNA splicing as corecomponent of the SMN- Sm complex thatmediates spliceosomal snRNP assembly andas component of the spliceosomal U1, U2,U4 and U5 small nuclear ribonucleoproteins(snRNPs), the building blocks of thespliceosome (PubMed: ,PubMed: ,PubMed: ,PubMed: ,PubMed: ,PubMed: ,PubMed: ,PubMed: ,PubMed: ).Component of both the pre-catalyticspliceosome B complex and activatedspliceosome C complexes (PubMed: ,PubMed: ,PubMed: ,PubMed: ,PubMed: ). Is also acomponent of the minor U12 spliceosome(PubMed: ). May actas a charged protein scaffold to promotesnRNP assembly or strengthensnRNP-snRNP interactions throughnon-specific electrostatic contacts with RNA(Probable).
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(SNRPD1 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(SNRPD1 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with SNRPD1 Antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with SNRPD1 Antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(All lanes : Anti-SNRPD1 Antibody (Center) at 1:1000 dilution Lane 1: HL-60 whole celll ysate Lane 2: K562 whole cell lysate. Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution. Predicted band size : 13 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-SNRPD1 Antibody (Center) at 1:1000 dilution Lane 1: HL-60 whole celll ysate Lane 2: K562 whole cell lysate. Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution. Predicted band size : 13 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-SNRPD1 antibody
SNRPD1 is a small nuclear ribonucleoprotein that belongs to the SNRNP core protein family. This protein may act as a charged protein scaffold to promote SNRNP assembly or strengthen SNRNP-SNRNP interactions through nonspecific electrostatic contacts with RNA.
Product Categories/Family for anti-SNRPD1 antibody
References
Lehner,B., Genome Res. 14 (7), 1315-1323 (2004)
Will,C.L., RNA 10 (6), 929-941 (2004)
Will,C.L., RNA 10 (6), 929-941 (2004)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13282
NCBI Official Full Name
small nuclear ribonucleoprotein Sm D1 isoform 1
NCBI Official Synonym Full Names
small nuclear ribonucleoprotein D1 polypeptide 16kDa
NCBI Official Symbol
SNRPD1
NCBI Official Synonym Symbols
SMD1; SNRPD; Sm-D1; HsT2456
NCBI Protein Information
small nuclear ribonucleoprotein Sm D1
UniProt Protein Name
Small nuclear ribonucleoprotein Sm D1
UniProt Gene Name
SNRPD1
UniProt Synonym Gene Names
Sm-D1
UniProt Entry Name
SMD1_HUMAN
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Product Notes
The SNRPD1 snrpd1 (Catalog #AAA283980) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 46-78. The SNRPD1 Antibody (Center) reacts with Human Predicted Reactivity: Bovine, Monkey, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SNRPD1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the SNRPD1 snrpd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SNRPD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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