IHC (Immunohiostchemistry)
(Immunohistochemistry analysis using Rabbit Anti-SOD2 Polyclonal Antibody (SPC-117). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-SOD2 Polyclonal Antibody (SPC-117) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Mitochondrion matrix.)
Rabbit SOD (Mn) Polyclonal Antibody | anti-Sod2 antibody
SOD (Mn) Antibody
Reactivity
Human, Rat, Mouse, Bovine, Canine, Chicken, Dosophila, Guinea pig, Pig, Hamster, Monkey, Rabbit, Sheep, Xenopus
Applications
Immunofluorescence, Immunohistochemistry, ELISA, Immunoprecipitation, Western Blot
Synonyms
SOD (Mn), Antibody; SOD (Mn) Antibody; Manganese SOD; IPO B; Mn SOD; SOD2; anti-Sod2 antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse, Bovine, Canine, Chicken, Dosophila, Guinea pig, Pig, Hamster, Monkey, Rabbit, Sheep, Xenopus
Clonality
Polyclonal
Form/Format
Affinity (antigen) Purified
Concentration
1mg/mL (varies by lot)
Sequence Length
222
Applicable Applications for anti-Sod2 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, IP (Immunoprecipitation), WB (Western Blot)
Certificate of Analysis
0.5 ug/mL of SPC-117 was sufficient for detection of Mn SOD in 20ug of rat brain tissue extract by colorimetric immunoblot analysis using Goat anti-rabbit IgG:AP as the secondary antibody.
Conjugate
Unconjugated
Storage Buffer
PBS pH7, 50% glycerol, 0.09% sodium azide
Preparation and Storage
-20 degree C
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis using Rabbit Anti-SOD2 Polyclonal Antibody (SPC-117). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-SOD2 Polyclonal Antibody (SPC-117) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Mitochondrion matrix.)
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis using Rabbit Anti-SOD2 Polyclonal Antibody (SPC-117). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-SOD2 Polyclonal Antibody (SPC-117) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Mitochondrion matrix.)
WB (Western Blot)
(Western blot analysis of Rat Tissue lysates showing detection of SOD2 protein using Rabbit Anti-SOD2 Polyclonal Antibody (SPC-117). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-SOD2 Polyclonal Antibody (SPC-117) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)
WB (Western Blot)
(Western blot analysis of Rat Tissue lysates showing detection of SOD2 protein using Rabbit Anti-SOD2 Polyclonal Antibody (SPC-117). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-SOD2 Polyclonal Antibody (SPC-117) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)
Related Product Information for anti-Sod2 antibody
Background Info: Detects a ~25kDa protein corresponding to the molecular mass of Mn superoxide dismutase (SOD) on SDS PAGE immunoblots.
Scientific Background: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2" to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (7). SOD3 can also be distinguished by its heparin-binding capacity (1).
Scientific Background: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2" to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (7). SOD3 can also be distinguished by its heparin-binding capacity (1).
Product Categories/Family for anti-Sod2 antibody
References
1.Adachi T., et al. (1992). Clin. Chim. Acta. 212: 89-102. 2.Barrister J.V., et al. (1987). Crit. Rev. Biochem. 22:111- 180. 3.Furukawa Y., O'Halloran T. (2006). Antioxidants & Redo Signaling. Vol 8, No 5,6. 4.Gao B., et al. (2003). Am J Physiol Lung Cell Mol Physiol 284: L917-L925. 5.Hassan H.M. (1988). Free Radical Biol. Med. 5: 377-385. 6.Kurobe N., et al. (1990) Biomedical Research. 11: 187- 194 7.Wispe J.R., et al. (1989) BBA. 994: 30-36. 8.Xiao-Hong Liu., et al. (1993) Brain Research. 625: 29-37.
1. Allan, M. E. and Storey, K. B. (2012). Expression of NF-κB and downstream antioxidant genes in skeletal muscle of hibernating ground squirrels, Spermophilus tridecemlineatus. Cell Biochemistry and Function. 30 (2), 166-174. doi:10.1002/cbf.1832
2. Ishii, H., Kunihiro, S., Tanaka, M., Hatano, K. and Nishikata, T. (2012). Cytosolic subunits of ATP synthase are localized to the cortical endoplasmic reticulum-rich domain of the ascidian egg myoplasm. Development, growth & differentiation. 54 (8), 753-766. doi: 10.1111/dgd.12003
3. Wu, H., Hsiao, T., Chien, C., and Lai, M. (2009). Ischemic conditioning by short periods of reperfusion attenuates renal ischemia/reperfusion induced apoptosis and autophagy in the rat. J Biomed Sci. 16 (19). doi:10.1186/1423-0127-16-19
1. Allan, M. E. and Storey, K. B. (2012). Expression of NF-κB and downstream antioxidant genes in skeletal muscle of hibernating ground squirrels, Spermophilus tridecemlineatus. Cell Biochemistry and Function. 30 (2), 166-174. doi:10.1002/cbf.1832
2. Ishii, H., Kunihiro, S., Tanaka, M., Hatano, K. and Nishikata, T. (2012). Cytosolic subunits of ATP synthase are localized to the cortical endoplasmic reticulum-rich domain of the ascidian egg myoplasm. Development, growth & differentiation. 54 (8), 753-766. doi: 10.1111/dgd.12003
3. Wu, H., Hsiao, T., Chien, C., and Lai, M. (2009). Ischemic conditioning by short periods of reperfusion attenuates renal ischemia/reperfusion induced apoptosis and autophagy in the rat. J Biomed Sci. 16 (19). doi:10.1186/1423-0127-16-19
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24,674 Da
NCBI Official Full Name
superoxide dismutase
NCBI Official Synonym Full Names
superoxide dismutase 2, mitochondrial
NCBI Official Symbol
Sod2
NCBI Protein Information
superoxide dismutase [Mn], mitochondrial; superoxide dismutase [Mn], mitochondrial; Superoxide dimutase 2, mitochondrial; mitochondrial superoxide dismutase 2
UniProt Protein Name
Superoxide dismutase [Mn], mitochondrial
UniProt Gene Name
Sod2
UniProt Entry Name
SODM_RAT
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Product Notes
The Sod2 sod2 (Catalog #AAA103682) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SOD (Mn) Antibody reacts with Human, Rat, Mouse, Bovine, Canine, Chicken, Dosophila, Guinea pig, Pig, Hamster, Monkey, Rabbit, Sheep, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's SOD (Mn) can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the Sod2 sod2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SOD (Mn), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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