IHC (Immunohistochemisry)
(Dilution: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (Sodium hydrogen exchanger 1) Polyclonal Antibody, Unconjugated (bs-0505R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
Rabbit Sodium hydrogen exchanger 1 Polyclonal Antibody | anti-SLC9A1 antibody
Sodium hydrogen exchanger 1 Polyclonal Antibody
Gene Names
SLC9A1; APNH; NHE1; NHE-1; PPP1R143
Reactivity
Rat, Pig, Dog, Cow
Applications
Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Sodium hydrogen exchanger 1, Antibody; Sodium hydrogen exchanger 1 Polyclonal Antibody; Sodium/hydrogen exchanger 1; APNH; Na (+)/H (+) antiporter; amiloride-sensitive; Na (+)/H (+) exchanger 1; NHE-1; Solute carrier family 9 member 1; SLC9A1; APNH1; NHE1; anti-SLC9A1 antibody
Host
Rabbit
Reactivity
Rat, Pig, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Applicable Applications for anti-SLC9A1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(Dilution: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (Sodium hydrogen exchanger 1) Polyclonal Antibody, Unconjugated (bs-0505R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
IHC (Immunohistochemisry)
(Dilution: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (Sodium hydrogen exchanger 1) Polyclonal Antibody, Unconjugated (bs-0505R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
WB (Western Blot)
(Sample:molt-4(human)cell Lysate at 40 ugPrimary: Anti- Sodium hydrogen exchanger 1 (bs-0505R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 91kDObserved band size: 91 kD)
WB (Western Blot)
(Sample:molt-4(human)cell Lysate at 40 ugPrimary: Anti- Sodium hydrogen exchanger 1 (bs-0505R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 91kDObserved band size: 91 kD)
WB (Western Blot)
(Sample:Brain(Rat) lysate at 30ug;Liver(Rat) lysate at 30ug;Primary: Anti-NHE1 (bs-0505R) at 1:200 dilution;Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000 dilution;Predicted band size : 91kDObserved band size : 91kDWe are unsure as to the identity of these extra bands)
WB (Western Blot)
(Sample:Brain(Rat) lysate at 30ug;Liver(Rat) lysate at 30ug;Primary: Anti-NHE1 (bs-0505R) at 1:200 dilution;Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000 dilution;Predicted band size : 91kDObserved band size : 91kDWe are unsure as to the identity of these extra bands)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
61,013 Da
NCBI Official Full Name
Sodium/hydrogen exchanger 1
NCBI Official Synonym Full Names
solute carrier family 9, subfamily A (NHE1, cation proton antiporter 1), member 1
NCBI Official Symbol
SLC9A1
NCBI Official Synonym Symbols
APNH; NHE1; NHE-1; PPP1R143
NCBI Protein Information
sodium/hydrogen exchanger 1; Na(+)/H(+) exchanger 1; Na-Li countertransporter; protein phosphatase 1, regulatory subunit 143; solute carrier family 9 (sodium/hydrogen exchanger), member 1 (antiporter, Na+/H+, amiloride sensitive); solute carrier family 9
UniProt Protein Name
Sodium/hydrogen exchanger 1
UniProt Gene Name
SLC9A1
UniProt Synonym Gene Names
APNH1; NHE1; NHE-1
UniProt Entry Name
SL9A1_HUMAN
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Product Notes
The SLC9A1 slc9a1 (Catalog #AAA291268) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Sodium hydrogen exchanger 1 Polyclonal Antibody reacts with Rat, Pig, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's Sodium hydrogen exchanger 1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the SLC9A1 slc9a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sodium hydrogen exchanger 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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