Rabbit Sodium Potassium ATPase alpha 1 Polyclonal Antibody | anti-ATP1A1 antibody

Phospho-Sodium Potassium ATPase alpha 1 (Tyr542) Antibody

Cat. Num. AAA328366

• Gene Names: ATP1A1; CMT2DD; HOMGSMR2
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
• Synonyms: Sodium Potassium ATPase alpha 1, Antibody; Phospho-Sodium Potassium ATPase alpha 1 (Tyr542) Antibody; ATPase Na+/K+ transporting alpha; adenosinetriphosphatase; AT1A1_HUMAN; ATP1A1; ATP1A4; ATP1AL2; ATP1B; ATP1B1; ATPase Na+/K+ transporting alpha 1 polypeptide; ATPase Na+/K+ transporting alpha 4 polypeptide; ATPase Na+/K+ transporting beta 1 polypeptide; ATPase, Na+/K+ transporting, alpha polypeptide-like 2; ATPase, Na+/K+ transporting, beta 1 polypeptide; Beta 1-subunit of Na(+),K(+)-ATPase; Na(+)/K(+) ATPase alpha-1 subunit; Na(+)/K(+) ATPase alpha-4 subunit; Na+, K+ ATPase alpha subunit; Na+/K+ ATPase 1; Na+/K+ ATPase 4; Na+/K+ ATPase, alpha-D polypeptide; Na, K-ATPase beta-1 polypeptide; Na, K-ATPase, alpha-A catalytic polypeptide; Na,K-ATPase catalytic subunit alpha-A protein; Na,K-ATPase subunit alpha-C; polypeptide-like 2; Sodium pump 1; sodium pump 4; Sodium pump subunit alpha-1; sodium pump subunit alpha-4; sodium pump subunit beta-1; sodium-potassium ATPase catalytic subunit alpha-1; sodium-potassium ATPase catalytic subunit alpha-4; sodium-potassium ATPase subunit beta 1 (non-catalytic); sodium-potassium ATPase, alpha 4 polypeptide; sodium-potassium-ATPase, alpha 1 polypeptide; Sodium/potassium transporting ATPase alpha 1 chain; Sodium/potassium transporting ATPase subunit beta 1; sodium/potassium-dependent ATPase beta-1 subunit; sodium/potassium-transporting ATPase alpha-4 chain; sodium/potassium-transporting ATPase beta-1 chain; Sodium/potassium-transporting ATPase subunit alpha-1; sodium/potassium-transporting ATPase subunit alpha-4; anti-ATP1A1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Phospho-ATP1 alpha1/Na+K+ ATPase1 (Tyr542) Antibody detects endogenous levels of ATP1 alpha1/Na+K+ ATPase1 only when phosphorylated at Tyr542.
• Purity/Purification: Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 1023

• Applicable Applications for anti-ATP1A1 antibody: ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human ATP1 alpha1/Na+K+ ATPase1 around the phosphorylation site of Tyr542.
• Subcellular Location: Plasma Membrane
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

WB (Western Blot)

(Western blot analysis of extracts of Rat brain tissue sample,using Phospho-ATP1 alpha1/Na+K+ ATPase1 (Tyr542) Antibody.)

IHC (Immunohistochemistry)

(At 1/100 staining mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

Related Product Information for anti-ATP1A1 antibody

Description: This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

Post Translational Modifications: Phosphorylation on Tyr-10 modulates pumping activity. Phosphorylation of Ser-943 by PKA modulates the response of ATP1A1 to PKC. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity (By similarity).

Subunit Structure: The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit. Interacts with regulatory subunit FXYD1 (By similarity). Interacts with regulatory subunit FXYD3 (PubMed:21454534). Interacts with SIK1 (By similarity). Binds the HLA class II histocompatibility antigen DR1 (PubMed:1380674). Interacts with SLC35G1 and STIM1 (PubMed:22084111).

Similarity: Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 21361181
• NCBI GeneID: 476
• NCBI Accession #: NP_000692.2
• NCBI GenBank Nucleotide #: NP_000692.2
• UniProt Accession #: P05023
• Molecular Weight: Observed: 113 kDa; Predicted: 113 kDa
• NCBI Official Full Name: sodium/potassium-transporting ATPase subunit alpha-1 isoform a
• NCBI Official Synonym Full Names: ATPase Na+/K+ transporting subunit alpha 1
• NCBI Official Symbol: ATP1A1
• NCBI Official Synonym Symbols: CMT2DD; HOMGSMR2
• NCBI Protein Information: sodium/potassium-transporting ATPase subunit alpha-1
• UniProt Protein Name: Sodium/potassium-transporting ATPase subunit alpha-1
• UniProt Gene Name: ATP1A1
• UniProt Synonym Gene Names: Na(+)/K(+) ATPase alpha-1 subunit
• UniProt Entry Name: AT1A1_HUMAN