IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with SOX18 Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SOX18 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).SOX18 immunoreactivity is localized to Nucleus significantly.)
Rabbit anti-Human SOX18 Polyclonal Antibody | anti-SOX18 antibody
SOX18 Antibody (N-term)
Gene Names
SOX18; HLTS; HLTRS
Reactivity
Human
Applications
Western Blot, ELISA, Immunofluorescence
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SOX18, Antibody; SOX18 Antibody (N-term); Transcription factor SOX-18; SOX18; anti-SOX18 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This SOX18 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-88 amino acids from the N-terminal region of human SOX18.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
59-88
Sequence Length
384
Applicable Applications for anti-SOX18 antibody
WB (Western Blot), ELISA, IF (Immunofluorescence)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with SOX18 Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SOX18 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).SOX18 immunoreactivity is localized to Nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with SOX18 Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SOX18 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).SOX18 immunoreactivity is localized to Nucleus significantly.)
WB (Western Blot)
(SOX18 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the SOX18 antibody detected the SOX18 protein (arrow).)
WB (Western Blot)
(SOX18 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the SOX18 antibody detected the SOX18 protein (arrow).)
Related Product Information for anti-SOX18 antibody
This gene encodes a member of the SOX (SRY-related
HMG-box) family of transcription factors involved in the regulation
of embryonic development and in the determination of the cell fate.
The encoded protein may act as a transcriptional regulator after
forming a protein complex with other proteins. This protein plays a
role in hair, blood vessel, and lymphatic vessel development.
Mutations in this gene have been associated with recessive and
dominant forms of hypotrichosis-lymphedema-telangiectasia.
HMG-box) family of transcription factors involved in the regulation
of embryonic development and in the determination of the cell fate.
The encoded protein may act as a transcriptional regulator after
forming a protein complex with other proteins. This protein plays a
role in hair, blood vessel, and lymphatic vessel development.
Mutations in this gene have been associated with recessive and
dominant forms of hypotrichosis-lymphedema-telangiectasia.
Product Categories/Family for anti-SOX18 antibody
References
Petrovic, I., et al. Mol. Biol. Rep. 36(5):993-1000(2009)
Fontijn, R.D., et al. Am. J. Physiol. Heart Circ. Physiol. 294 (2), H891-H900 (2008) :
Ferrell, R.E., et al. Lymphat Res Biol 6(2):69-76(2008)
Finegold, D.N., et al. Lymphat Res Biol 6(2):65-68(2008)
Young, N., et al. J. Natl. Cancer Inst. 98(15):1060-1067(2006)
Fontijn, R.D., et al. Am. J. Physiol. Heart Circ. Physiol. 294 (2), H891-H900 (2008) :
Ferrell, R.E., et al. Lymphat Res Biol 6(2):69-76(2008)
Finegold, D.N., et al. Lymphat Res Biol 6(2):65-68(2008)
Young, N., et al. J. Natl. Cancer Inst. 98(15):1060-1067(2006)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40891
NCBI Official Full Name
transcription factor SOX-18
NCBI Official Synonym Full Names
SRY (sex determining region Y)-box 18
NCBI Official Symbol
SOX18
NCBI Official Synonym Symbols
HLTS; HLTRS
NCBI Protein Information
transcription factor SOX-18
UniProt Protein Name
Transcription factor SOX-18
UniProt Gene Name
SOX18
UniProt Entry Name
SOX18_HUMAN
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Product Notes
The SOX18 sox18 (Catalog #AAA289632) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 59-88. The SOX18 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SOX18 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, IF (Immunofluorescence). Researchers should empirically determine the suitability of the SOX18 sox18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SOX18, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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