WB (Western Blot)
(Detection of human SP1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, MCF-7, and CCRF-CEM cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SP1 antibody (AAA210780 lot 6) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse Sp1 Polyclonal Antibody | anti-Sp1 antibody
Rabbit anti-Sp1 Antibody, Affinity Purified
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human SP1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, MCF-7, and CCRF-CEM cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SP1 antibody (AAA210780 lot 6) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human SP1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, MCF-7, and CCRF-CEM cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SP1 antibody (AAA210780 lot 6) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human SP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SP1 antibody (AAA210780 lot 6) used for IP at 6 ug per reaction. SP1 was also immunoprecipitated by a previous lot of this antibody (AAA210780 lot 5) and a second antibody against a different epitope of SP1 . For blotting immunoprecipitated SP1, AAA210780 was used at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human SP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SP1 antibody (AAA210780 lot 6) used for IP at 6 ug per reaction. SP1 was also immunoprecipitated by a previous lot of this antibody (AAA210780 lot 5) and a second antibody against a different epitope of SP1 . For blotting immunoprecipitated SP1, AAA210780 was used at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemisry)
(Detection of human SP1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-SP1 (AAA210780) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemisry)
(Detection of human SP1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-SP1 (AAA210780) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of mouse SP1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-SP1 (AAA210780) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of mouse SP1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-SP1 (AAA210780) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
ChIP (Chromatin Immunoprecipitation)
(Localization of Sp1 Binding Sites by ChIP-sequencing. Chromatin from acute myeloid leukemia cell line was immunoprecipitated with anti-Sp1 antibody AAA210780 and analyzed by DNA sequencing. The figure illustrates the peak distribution of Sp1 binding within a 250 Kb region of chromosome 6 as detected using anti-Sp1 AAA210780. ChIP-seq validation performed by Active Motif, Carlsbad, CA.)
ChIP (Chromatin Immunoprecipitation)
(Localization of Sp1 Binding Sites by ChIP-sequencing. Chromatin from acute myeloid leukemia cell line was immunoprecipitated with anti-Sp1 antibody AAA210780 and analyzed by DNA sequencing. The figure illustrates the peak distribution of Sp1 binding within a 250 Kb region of chromosome 6 as detected using anti-Sp1 AAA210780. ChIP-seq validation performed by Active Motif, Carlsbad, CA.)
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Product Notes
The Sp1 sp1 (Catalog #AAA210780) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Sp1 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Sp1 can be used in a range of immunoassay formats including, but not limited to, ChIP (Chromatin immunoprecipitation), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the Sp1 sp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sp1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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