DB (Dot Blot)
(Sp1 phospho Ser101 antibody tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Sp1 phospho Ser101 antibody for Sp1 phosphorylated at serine 101. Modified and unmodified peptides were spotted onto PVDF and probed with Sp1 phospho Ser101 antibody at a dilution of 1:5,000. The amount of peptide spotted (in picomoles) is indicated next to each row. Lane 1: Unmodified Sp1 peptide. Lane 2: Sp1 peptide phosphorylated at Serine 101.)
Rabbit anti-Human Sp1 phospho Ser101 Polyclonal Antibody | anti-Sp1 antibody
Sp1 phospho Ser101 antibody (pAb)
Reactivity
Human
Applications
Western Blot
Purity
Affinity Purified
Synonyms
Sp1 phospho Ser101, Antibody; Sp1 phospho Ser101 antibody (pAb); TSFP1; anti-Sp1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-Sp1 antibody
WB (Western Blot)
Conjugation
Phosphorylated
Immunogen
This Sp1 phospho Ser101 antibody was raised against a peptide containing phospho-serine 101 of human Sp1.
Abs Residue
Ser
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
DB (Dot Blot)
(Sp1 phospho Ser101 antibody tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Sp1 phospho Ser101 antibody for Sp1 phosphorylated at serine 101. Modified and unmodified peptides were spotted onto PVDF and probed with Sp1 phospho Ser101 antibody at a dilution of 1:5,000. The amount of peptide spotted (in picomoles) is indicated next to each row. Lane 1: Unmodified Sp1 peptide. Lane 2: Sp1 peptide phosphorylated at Serine 101.)
DB (Dot Blot)
(Sp1 phospho Ser101 antibody tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Sp1 phospho Ser101 antibody for Sp1 phosphorylated at serine 101. Modified and unmodified peptides were spotted onto PVDF and probed with Sp1 phospho Ser101 antibody at a dilution of 1:5,000. The amount of peptide spotted (in picomoles) is indicated next to each row. Lane 1: Unmodified Sp1 peptide. Lane 2: Sp1 peptide phosphorylated at Serine 101.)
WB (Western Blot)
(Sp1 phospho Ser101 antibody tested by Western blot. Sp1 phospho Ser101 was detected in nuclear extracts of MCF-7 cells (25 ug per lane) using Sp1 phospho Ser101 antibody at dilution of 1:500. Lane 1: No treatment. Lane 2: Cells treated with H2O2.)
WB (Western Blot)
(Sp1 phospho Ser101 antibody tested by Western blot. Sp1 phospho Ser101 was detected in nuclear extracts of MCF-7 cells (25 ug per lane) using Sp1 phospho Ser101 antibody at dilution of 1:500. Lane 1: No treatment. Lane 2: Cells treated with H2O2.)
Related Product Information for anti-Sp1 antibody
Short Description: Our Sp1 phospho Ser101 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot; it has been shown to react with Human samples.
Background: Sp1 (specificity protein 1) is a human transcription factor involved in gene expression in the early development of an organism. The protein is 785 amino acids long, with a molecular weight of 81 kDa. The SP1 transcription factor contains a zinc finger protein motif, by which it binds directly to DNA and enhances gene transcription. Its zinc fingers are of the Cys2/His2 type and bind the consensus sequence 5'-(G/T)GGGCGG(G/A)(G/A)(C/T)-3' (GC box element). It was discovered in 1983 and has since been modified to form Sp1C, which has a zinc finger protein DNA binding domain. Sp1 contains two SQ/TQ domains, which are found in proteins phosphorylated by the DNA-damage response transducer/effector kinase ATM. Phosphorylation of serine 101 (dependent upon ATM and ATR) is stimulated by double strand DNA breakage (DSBs) as well as by blocks to DNA replication induced by treatment with hydroxyurea or UV irradiation. Sp1 phospho Ser101 co-localizes with ATM phospho Ser1981 at DSBs, suggesting that this phosphorylated version of Sp1 plays a role in double strand break repair.
Background: Sp1 (specificity protein 1) is a human transcription factor involved in gene expression in the early development of an organism. The protein is 785 amino acids long, with a molecular weight of 81 kDa. The SP1 transcription factor contains a zinc finger protein motif, by which it binds directly to DNA and enhances gene transcription. Its zinc fingers are of the Cys2/His2 type and bind the consensus sequence 5'-(G/T)GGGCGG(G/A)(G/A)(C/T)-3' (GC box element). It was discovered in 1983 and has since been modified to form Sp1C, which has a zinc finger protein DNA binding domain. Sp1 contains two SQ/TQ domains, which are found in proteins phosphorylated by the DNA-damage response transducer/effector kinase ATM. Phosphorylation of serine 101 (dependent upon ATM and ATR) is stimulated by double strand DNA breakage (DSBs) as well as by blocks to DNA replication induced by treatment with hydroxyurea or UV irradiation. Sp1 phospho Ser101 co-localizes with ATM phospho Ser1981 at DSBs, suggesting that this phosphorylated version of Sp1 plays a role in double strand break repair.
Product Categories/Family for anti-Sp1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
75,824 Da
NCBI Official Full Name
transcription factor Sp1 isoform a
NCBI Official Synonym Full Names
Sp1 transcription factor
NCBI Official Symbol
SP1
NCBI Protein Information
transcription factor Sp1
UniProt Protein Name
Transcription factor Sp1
UniProt Gene Name
SP1
UniProt Synonym Gene Names
TSFP1
UniProt Entry Name
SP1_HUMAN
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Product Notes
The Sp1 sp1 (Catalog #AAA59927) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Sp1 phospho Ser101 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Sp1 phospho Ser101 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the Sp1 sp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sp1 phospho Ser101, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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