IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using Spata5 Rabbit pAb (AAA282381) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)
Rabbit Spata5 Polyclonal Antibody | anti-Spata5 antibody
Spata5 Rabbit pAb
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Affinity purification
Synonyms
Spata5, Antibody; Spata5 Rabbit pAb; Spaf; 2510048F20Rik; anti-Spata5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
Liquid
PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Applicable Applications for anti-Spata5 antibody
ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Positive Samples
Mouse testis, Rat testis
Immunogen
Recombinant protein of Mouse Spata5.
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Ship: Ice Pack
Ship: Ice Pack
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using Spata5 Rabbit pAb (AAA282381) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using Spata5 Rabbit pAb (AAA282381) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using Spata5 antibody (AAA282381) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using Spata5 antibody (AAA282381) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.)
Related Product Information for anti-Spata5 antibody
Predicted to enable ATP hydrolysis activity. Predicted to be involved in brain development. Predicted to act upstream of or within cell differentiation and spermatogenesis. Predicted to be located in spindle. Predicted to be active in cytoplasm. Is expressed in several structures, including cartilage; endocrine gland; genitourinary system; nervous system; and trachea. Orthologous to human SPATA5 (spermatogenesis associated 5).
Product Categories/Family for anti-Spata5 antibody
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Product Notes
The Spata5 (Catalog #AAA282381) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Spata5 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Spata5 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the Spata5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Spata5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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