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IF (Immunofluorescence)
(Confocal immunofluorescent analysis of SPECC1 Antibody (Center) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
Rabbit SPECC1 Polyclonal Antibody | anti-SPECC1L antibody
SPECC1 Antibody (Center)
Gene Names
SPECC1L; CYTSA; GBBB2; OBLFC1
Reactivity
Human, mouse (Predicted Reactivity: Chicken, Zebrafish)
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SPECC1, Antibody; SPECC1 Antibody (Center); Cytospin-A; Renal carcinoma antigen NY-REN-22; Sperm antigen with calponin homology and coiled-coil domains 1-like; SPECC1-like protein; SPECC1L; CYTSA; KIAA0376; anti-SPECC1L antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Chicken, Zebrafish)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This SPECC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 705-733 amino acids from the Central region of human SPECC1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.48 (varies by lot)
Sequence Positions
705-733
Sequence Length
1078
Applicable Applications for anti-SPECC1L antibody
IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of SPECC1 Antibody (Center) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of SPECC1 Antibody (Center) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
FCM/FACS (Flow Cytometry)
(SPECC1 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(SPECC1 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemisry)
(SPECC1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SPECC1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(SPECC1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SPECC1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(SPECC1 Antibody (Center) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the SPECC1 antibody detected the SPECC1 protein (arrow).)
WB (Western Blot)
(SPECC1 Antibody (Center) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the SPECC1 antibody detected the SPECC1 protein (arrow).)
WB (Western Blot)
(SPECC1 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the SPECC1 antibody detected the SPECC1 protein (arrow).)
WB (Western Blot)
(SPECC1 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the SPECC1 antibody detected the SPECC1 protein (arrow).)
Related Product Information for anti-SPECC1L antibody
CYTSAis involved in cytokinesis and spindle organization.
Product Categories/Family for anti-SPECC1L antibody
References
Scanlan, M.J., et al. Int. J. Cancer 83(4):456-464(1999)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
124602
NCBI Official Full Name
cytospin-A isoform 2
NCBI Official Synonym Full Names
sperm antigen with calponin homology and coiled-coil domains 1-like
NCBI Official Symbol
SPECC1L
NCBI Official Synonym Symbols
CYTSA; GBBB2; OBLFC1
NCBI Protein Information
cytospin-A
UniProt Protein Name
Cytospin-A
UniProt Gene Name
SPECC1L
UniProt Synonym Gene Names
CYTSA; KIAA0376; SPECC1-like protein
UniProt Entry Name
CYTSA_HUMAN
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Product Notes
The SPECC1L specc1l (Catalog #AAA288000) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 705-733. The SPECC1 Antibody (Center) reacts with Human, mouse (Predicted Reactivity: Chicken, Zebrafish) and may cross-react with other species as described in the data sheet. AAA Biotech's SPECC1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the SPECC1L specc1l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPECC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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