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IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Liver Tissue))
Rabbit anti-Human, Rat Sperm Associated Antigen 1 Polyclonal Antibody | anti-SPAG1 antibody
Sperm Associated Antigen 1 (SPAG1) Polyclonal Antibody
Gene Names
SPAG1; SP75; TPIS; CT140; CILD28; HSD-3.8; HEL-S-268
Reactivity
Human, Rat
Applications
ELISA, Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Affinity Chromatography
Synonyms
Sperm Associated Antigen 1, Antibody; Sperm Associated Antigen 1 (SPAG1) Polyclonal Antibody; HSD-3.8; SP75; TPIS; anti-SPAG1 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against SPAG1. It has been selected for its ability to recognize SPAG1 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence Length
926
Applicable Applications for anti-SPAG1 antibody
ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot)
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Human, Rat
Organism Species
Human
Fragment
SPAG1 (Met1~Lys365)
Quality Control
Content: The quality control contains recombinant SPAG1 (Met1~Lys365) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Liver Tissue))
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Liver Tissue))
IHC (Immunohistochemisry)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
IHC (Immunohistochemisry)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
WB (Western Blot)
(Western Blot: Sample: Recombinant protein.)
WB (Western Blot)
(Western Blot: Sample: Recombinant protein.)
WB (Western Blot)
(Western Blot: Sample: Human Jurkat cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human SPAG1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot: Sample: Human Jurkat cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human SPAG1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,031 Da
NCBI Official Full Name
sperm-associated antigen 1
NCBI Official Synonym Full Names
sperm associated antigen 1
NCBI Official Symbol
SPAG1
NCBI Official Synonym Symbols
SP75; TPIS; CT140; CILD28; HSD-3.8; HEL-S-268
NCBI Protein Information
sperm-associated antigen 1
UniProt Protein Name
Sperm-associated antigen 1
UniProt Gene Name
SPAG1
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Product Notes
The SPAG1 spag1 (Catalog #AAA145237) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Sperm Associated Antigen 1 (SPAG1) Polyclonal Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Sperm Associated Antigen 1 can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the SPAG1 spag1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sperm Associated Antigen 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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