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IF (Immunofluorescence)
(Immunofluorescent analysis of HepG2 cells using AAA232694 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Rabbit anti-Human, Mouse SPHK1 Polyclonal Antibody | anti-SPHK1 antibody
SPHK1 Antibody
Gene Names
SPHK1; SPHK
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
Purity
>95%, Protein G purified
Synonyms
SPHK1, Antibody; SPHK1 Antibody; Sphingosine kinase 1; SPHK1; SPHK; SPK; anti-SPHK1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
384
Applicable Applications for anti-SPHK1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
Recombinant human Sphingosine kinase 1 protein (1-398AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, PH 7.4
Conjugation
Non-conjugated
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-34528 / sc-48825
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
IF (Immunofluorescence)
(Immunofluorescent analysis of HepG2 cells using AAA232694 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IF (Immunofluorescence)
(Immunofluorescent analysis of HepG2 cells using AAA232694 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using AAA232694 at dilution of 1:100)
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using AAA232694 at dilution of 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human liver cancer tissue using AAA232694 at dilution of 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human liver cancer tissue using AAA232694 at dilution of 1:100)
WB (Western Blot)
(Western BlotPositive WB detected in:Mouse skeletal muscle tissueAll lanes: SPHK1 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 43,52,44 kDaObserved band size: 43 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in:Mouse skeletal muscle tissueAll lanes: SPHK1 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 43,52,44 kDaObserved band size: 43 kDa)
Related Product Information for anti-SPHK1 antibody
Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D, L-threo-dihydrosphingosine, N, N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,944 Da
NCBI Official Full Name
sphingosine kinase 1 isoform 3
NCBI Official Synonym Full Names
sphingosine kinase 1
NCBI Official Symbol
SPHK1
NCBI Official Synonym Symbols
SPHK
NCBI Protein Information
sphingosine kinase 1
UniProt Protein Name
Sphingosine kinase 1
UniProt Gene Name
SPHK1
UniProt Synonym Gene Names
SPHK; SPK; SK 1; SPK 1
UniProt Entry Name
SPHK1_HUMAN
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Product Notes
The SPHK1 sphk1 (Catalog #AAA232694) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPHK1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SPHK1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the SPHK1 sphk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPHK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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